Xerostomia, hyposalivation and sialadenitis in patients with chronic hepatitis C are not associated with the detection of HCV RNA in saliva or salivary glands
Xerostomia, hyposalivation and sialadenitis in patients with chronic hepatitis C are not associated with the detection of HCV RNA in saliva or salivary glands
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DOI：
10.1136/jcp.2010.080036
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发表时间：
2010-11-01
影响因子：
3.4
通讯作者：
Vieira do Carmo, Maria Auxiliadora
中科院分区：
文献类型：
--
作者：
Camargo Grossmann, Soraya de Mattos;Teixeira, Rosangela;Vieira do Carmo, Maria Auxiliadora
目的慢性丙型肝炎（CHC）患者的唾液腺紊乱被认为是口腔肝外表现，强化了丙型肝炎病毒（HCV）作为嗜唾液病毒的作用。因此，作者调查了CHC患者唾液和唾液腺中HCV RNA的流行及其与口干症、唾液分泌不足和涎腺炎的可能关联。采用巢式RT-PCR方法对65例确诊CHC患者的唾液和小唾液腺进行HCV RNA检测。口腔干燥，唾液分泌不足，临床和组织病理学证据涎腺炎也进行了评估。采用单因素和多因素分析来验证相关性。结果在26/65（40.0%）患者的唾液和12/65（18.5%）患者的唾液腺中检出HCV RNA。23/65（35.4%）的患者报告口干，13/65（20.0%）的患者诊断为唾液分泌不足。65例患者中有31例（47.7%）经组织病理学检查证实为腺炎。31例涎腺炎患者中有12例（38.7%）在唾液中出现HCV RNA， 2/31（6.5%）在唾液腺中出现HCV RNA。没有发现口干、唾液分泌不足或涎腺炎与唾液或唾液腺中HCV RNA的检测有关联。结论：虽然口干、唾液分泌不足和涎腺炎是CHC患者的常见症状，但我们的研究并未证实唾液或唾液腺中HCV RNA的检测与这些唾液腺疾病之间的关联。然而，不能排除HCV通过免疫介导的病毒机制在这组患者唾液腺疾病发病机制中的间接作用。
Objective Salivary gland disorders in patients with chronic hepatitis C (CHC) have been considered oral extrahepatic manifestations, reinforcing the hepatitis C virus (HCV) as a sialotropic virus. Hence, the authors investigated the prevalence of HCV RNA in saliva and salivary glands and its possible association with xerostomia, hyposalivation and sialadenitis in patients with CHC.Patients and methods In 65 patients with confirmed CHC, the HCV RNA was investigated by nested RT-PCR in saliva samples and minor salivary glands. Xerostomia, hyposalivation, clinical and histopathological evidence of sialadenitis were also evaluated. Univariate and multivariate analyses were employed to verify associations.Results HCV RNA was detected in the saliva of 26/65 (40.0%) patients and in 12/65 (18.5%) salivary glands. Xerostomia was reported by 23/65 (35.4%) patients, and hyposalivation was diagnosed in 13/65 (20.0%) patients. Sialadenitis was confirmed by histopathological features in 31/65 (47.7%) patients. Twelve (38.7%) of the 31 patients with sialadenitis presented HCV RNA in saliva and 2/31 (6.5%) in salivary glands. No associations were found between xerostomia, hyposalivation or sialadenitis and the detection of HCV RNA in saliva or in salivary glands.Conclusions Although xerostomia, hyposalivation and sialadenitis are frequent findings in CHC patients, our study did not confirm the association between the detection of HCV RNA in saliva or salivary glands with these salivary gland disorders. However, an indirect role of HCV by immune-mediated virus mechanisms in the pathogenesis of salivary gland disorders in this group of patients cannot be ruled out.