Cold-Induced CBF-PIF3 Interaction Enhances Freezing Tolerance by Stabilizing the phyB Thermosensor in Arabidopsis
Cold-Induced CBF-PIF3 Interaction Enhances Freezing Tolerance by Stabilizing the phyB Thermosensor in Arabidopsis
复制标题
寒冷诱导的 CBF-PIF3 相互作用通过稳定拟南芥中的 phyB 热传感器来增强冷冻耐受性
DOI：
10.1016/j.molp.2020.04.006
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发表时间：
2020-06-01
期刊：
影响因子：
27.5
通讯作者：
Yang, Shuhua
中科院分区：
文献类型：
--
作者：
Jiang, Bochen;Shi, Yiting;Yang, Shuhua
生长抑制和冷驯化策略有助于植物抵御冷胁迫，这对生长和存活产生不利影响。光敏色素B（phyB）通过感知光和环境温度信号来调节植物生长。然而，phyB介导植物对冷胁迫的反应的机制仍然是难以捉摸的。在这里，我们表明，关键的转录因子介导的冷驯化，C-重复结合因子（CBFs），与植物色素相互作用因子3（PIF 3）在冷胁迫下相互作用，从而削弱了相互确保破坏PIF 3-phyB。冷稳定phyB的CBFs的下游行为，积极调节抗冻性，通过调节应激反应和生长相关基因的表达。与此相一致，phyB突变体表现出冷冻敏感的表型，而phyB过表达转基因植物表现出增强的抗冻性。进一步的分析表明，PIF 1，PIF 4和PIF 5蛋白，所有这些负调节植物的抗冻性，不稳定的冷胁迫中的光敏色素依赖的方式。总的来说，我们的研究表明，CBFs-PIF 3-phyB作为一个重要的调节模块，调节植物对冷胁迫的反应。
Growth inhibition and cold-acclimation strategies help plants withstand cold stress, which adversely affects growth and survival. PHYTOCHROME B (phyB) regulates plant growth through perceiving both light and ambient temperature signals. However, the mechanism by which phyB mediates the plant response to cold stress remains elusive. Here, we show that the key transcription factors mediating cold acclimation, C-REPEAT BINDING FACTORs (CBFs), interact with PHYTOCHROME-INTERACTING FACTOR 3 (PIF3) under cold stress, thus attenuating the mutually assured destruction of PIF3-phyB. Cold-stabilized phyB acts downstream of CBFs to positively regulate freezing tolerance by modulating the expression of stressresponsive and growth-related genes. Consistent with this, phyB mutants exhibited a freezing-sensitive phenotype, whereas phyB-overexpression transgenic plants displayed enhanced freezing tolerance. Further analysis showed that the PIF1, PIF4, and PIF5 proteins, all of which negatively regulate plant freezing tolerance, were destabilized by cold stress in a phytochrome-dependent manner. Collectively, our study reveals that CBFs-PIF3-phyB serves as an important regulatory module for modulating plant response to cold stress.