Light responses from one type of ON-OFF amacrine cells in the rabbit retina.
Light responses from one type of ON-OFF amacrine cells in the rabbit retina.
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兔子视网膜中一种开关无长突细胞的光反应。
DOI：
10.1152/jn.1995.74.6.2460
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Raviola,E
中科院分区：
文献类型：
--
作者：
Dacheux,RF;Raviola,E
1. 在不同的光适应条件下，在灌流的兔视网膜中记录细胞内一种类型的开-关无长突细胞的光反应。这些记录是从位于中心区域的细胞获得的。视神经乳头正下方下方 5-7 毫米。 2.开关无长突细胞对光刺激的开始和终止做出短暂去极化反应。它们的感受野直径约为 0.8-1 毫米，并且没有表现出对抗性的中心和周围组织。 3. 细胞接受视杆细胞输入，因为它们对非常暗淡的暗视刺激有反应。随着暗适应时间的延长，细胞对刺激的开始变得比刺激的终止更敏感，因为光响应的“开”部分的阈值比“关”部分的阈值低。 4. 当视网膜适应视杆细胞饱和强度的背景照明时，细胞继续对测试闪光做出反应。因此，开关无长突细胞也接收视锥细胞输入。在这些明视条件下，在关闭分量之后观察到二次后电势。其特征与在兔子其他视网膜细胞中报道的视杆后效应的特征不同，因为其潜伏期和幅度随着刺激强度的增加而变化。 5. 辣根过氧化物酶的细胞内注射表明，记录是从一类开-关无长突细胞获得的，这些细胞的宽视野、单层树突被严格限制在内部丛状层或层3的中间。 6. 一类既不与杆双极连接也不与杆双极连接的无长突细胞中显着的杆和锥输入所有无长突细胞强烈支持这样的观点，即在兔子中，杆通路使用锥双极作为中间神经元，将暗视信号分配给神经节和视锥细胞驱动的无长突细胞。
1. The light responses from one type of ON-OFF amacrine cell were recorded intracellularly in the superfused rabbit retina under various conditions of light adaptation. These recordings were obtained from cells located in a central area. 5-7 mm inferior and directly below the optic nerve head. 2. ON-OFF amacrine cells responded to the initiation and termination of light stimuli with transient depolarizations. Their receptive fields were approximately 0.8-1 mm diam and did not exhibit antagonistic center-and-surround organization. 3. The cells received rod input because they responded to very dim scotopic stimuli. With prolonged dark adaptation, the cells became more sensitive to the initiation than termination of the stimulus, because the ON component of the light response had a lower threshold than the OFF component. 4. The cells continued to respond to test flashes when the retina was adapted to a background illumination of rod-saturating intensity. Thus ON-OFF amacrine cells also receive cone input. Under these photopic conditions, a secondary afterpotential was observed following the OFF component. Its characteristics were different from those of the rod aftereffect reported in other retinal cells of the rabbit because its latency and amplitude changed with increasing stimulus intensity. 5. Intracellular injections of horseradish peroxidase showed that the recordings were obtained from a class of ON-OFF amacrine cells whose wide-field, unistratified dendrites were rigorously confined to the middle of the inner plexiform layer or stratum 3. 6. The conspicuous rod and cone inputs into a class of amacrine cells that are connected neither to rod bipolars nor to All amacrine cells strongly support the idea that in the rabbit the rod pathway uses cone bipolars as interneurons to distribute scotopic signals to ganglion and cone-driven amacrine cells.