MUC1 expression and its association with other aetiological factors and localization to mitochondria in preneoplastic and neoplastic gastric tissues
MUC1 expression and its association with other aetiological factors and localization to mitochondria in preneoplastic and neoplastic gastric tissues
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DOI：
10.1016/j.cca.2010.09.003
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发表时间：
2010-12-14
影响因子：
5
通讯作者：
Devaraj, Halagowder
中科院分区：
文献类型：
--
作者：
Benjamin, J. B. Elisha;Jayanthi, Venkatraman;Devaraj, Halagowder
背景：人们对正常人胃上皮转化为腺癌的分子事件知之甚少。 MUC1 过度表达和线粒体定位可能会减弱癌细胞应激诱导的细胞凋亡。我们研究了癌前和癌变人胃组织中MUC1的表达模式、与HSP70的相互作用以及在线粒体中的定位。方法：采用免疫组织化学和Western印迹研究MUC1的表达模式和在线粒体中的定位。免疫共沉淀用于研究 MUC1 与 HSP70 的相互作用。 MUC1 表达与包括 erbB2 表达在内的其他致病特征相关。结果：MUC1 表达率为 75.8% (147/194)。 MUC1在50.0%（19/38例）不典型增生组织和58.2%（32/55例）腺癌组织中检测到过表达。在 71.66%（43/60 例）中观察到 MUC1-CT-HSP70 相互作用，在 33.33%（5/15）不典型增生样本和 47.05%（8/17）腺癌样本中 MUC1 定位于线粒体。 MUC1 表达与吸烟显着相关 (chi(2)=5.945；p
Background: The molecular events that underlie the conversion of normal human gastric epithelium into adenocarcinoma arc poorly understood. MUC1 overexpression and localization in mitochondria might confer cancer cells with attenuation of stress induced apoptosis. We studied MUC1 expression pattern, interaction with HSP70 and localization in mitochondria in preneoplastic and neoplastic human gastric tissues.Methods: lmmunohistochemistry and Western blot were used to study MUC1 expression pattern and localization in mitochondria. Coimmunoprecipitation was used to study MUC1 interaction with HSP70. MUC1 expression was correlated with other causative features including erbB2 expression.Results: MUC1 was expressed in 75.8% (147/194). MUC1 overexpression was detected in 50.0% (19/38 cases) dysplasia and 58.2% (32/55 cases) adenocarcinoma tissues. MUC1-CT-HSP70 interaction was seen in 71.66% (43/60 cases) and MUC1 localized to mitochondria in 33.33% (5/15) dysplasia samples and in 47.05% (8/17) adenocarcinoma samples. MUC1 expression showed significant association with smoking (chi(2)=5.945; p