Evaluation of different solvents on flavonoids extraction efficiency from sweet oranges and ripe and immature Seville oranges
Evaluation of different solvents on flavonoids extraction efficiency from sweet oranges and ripe and immature Seville oranges
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DOI：
10.1111/ijfs.14576
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发表时间：
2020-04-28
影响因子：
3.3
通讯作者：
Alvarez-Mateos, Paloma
中科院分区：
文献类型：
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作者：
Feng, Chao-Hui;Garcia-Martin, Juan Francisco;Alvarez-Mateos, Paloma
研究了不同乙醇（50%、80%、100%）和甲醇（50%、80%、100%）水溶液对甜橙（SO）皮、成熟苦橙（RBO）皮和未成熟苦橙（IBO）皮中黄酮类化合物提取效率的影响。与RBO和IBO相比，SO果皮中柚皮苷含量最高。UHPLC-HRMS/MS结果证实，3种橙子中均存在对香豆酸和奎宁酸，RBO和SO中咖啡酸的检出率分别为90.91%和20%。IBO、RBO和SO检测柚皮素的概率分别为100%、63.64%和40%。RBO中新橙皮苷和柚皮苷含量最高。建议用80%的甲醇提取黄酮类化合物，以获得最高的索氏得率和沉淀得率，而用100%的乙醇提取RBO和IBO中黄酮类化合物最合适。
The effects of different ethanol (50%, 80% and 100%) and methanol (50%, 80% and 100%) aqueous solutions on extraction efficiency of flavonoids from sweet orange (SO) peels, ripe bitter orange (RBO) and immature bitter orange (IBO) were investigated. The highest amount of naringin was found in SO peels, compared to RBO and IBO. UHPLC-HRMS/MS results confirmed the presence of p-coumaric acid and quinic acid in all the three types of oranges while there were 90.91% and 20% possibilities for detecting caffeic acid in RBO and SO, respectively. The probabilities of detecting naringenin from IBO, RBO and SO were 100%, 63.64% and 40%, respectively. The highest neohesperidin and naringin amounts were obtained in RBO. Using 80% methanol was recommended for extracting flavonoids from SO to obtain the highest Soxhlet and precipitation yields whereas 100% ethanol was the most suitable solvent to extract flavonoids from RBO and IBO.