EFFECTS OF CU-ZN SUPEROXIDE-DISMUTASE OVEREXPRESSION ON LIFE-SPAN AND RESISTANCE TO OXIDATIVE STRESS IN TRANSGENIC DROSOPHILA-MELANOGASTER
EFFECTS OF CU-ZN SUPEROXIDE-DISMUTASE OVEREXPRESSION ON LIFE-SPAN AND RESISTANCE TO OXIDATIVE STRESS IN TRANSGENIC DROSOPHILA-MELANOGASTER
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DOI：
10.1006/abbi.1993.1111
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发表时间：
1993-02-15
影响因子：
3.9
通讯作者：
SOHAL, RS
中科院分区：
文献类型：
--
作者：
ORR, WC;SOHAL, RS
由于活性氧能够造成分子损伤，因此它们被认为是衰老过程中的一个致病因素。铜锌超氧化物歧化酶（Cu-Zn SOD;超氧化物：超氧化物氧化还原酶; EC 1.15.1.1）清除超氧阴离子自由基，作为一个长寿决定因素的作用在转基因果蝇中进行了研究。将含有果蝇Cu-Zn SOD基因的基因组片段通过含P元件的Casper载体导入生殖细胞。在不同的转基因品系中，超氧化物歧化酶超过正常水平32-42%对果蝇的寿命及其耐受百草枯摄入和高氧（100%氧气）诱导的实验性氧化应激的能力有轻微和/或不显著的影响。转基因作物的平均寿命略有增加，但对过氧化氢的抗性也相应提高，但对百草枯的抗性没有提高。种群的最大寿命没有受到影响。
Reactive oxygen species have been postulated to be a causal factor in the aging process due to their ability to inflict molecular damage. The role of the enzyme copper-zinc superoxide dismutase (Cu-Zn SOD; superoxide:superoxide oxidoreductase; EC 1.15.1.1), which scavenges superoxide anion radicals, as a longevity determinant was examined in transgenicDrosophila melanogaster. A genomic fragment containing theDrosophilaCu-Zn SOD gene was introduced into the germ cells via a P element containing Casper vector. In different transgenic lines, overexpression of SOD by 32-42% above normal had either a minor and/or an insignificant effect on life span of the flies and their ability to withstand experimental oxidative stress, induced by paraquat intake and exposure to hyperoxia (100% oxygen). Transgenics showing a small increase in mean life span also exhibited a corresponding improvement in their resistance to byperoxia but not paraquat. The maximum life span of populations was not affected.