Immunolocalization of the plasma membrane Ca2+ pump isoforms in the rat brain
Immunolocalization of the plasma membrane Ca2+ pump isoforms in the rat brain
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DOI：
10.1016/s0006-8993(96)01282-6
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发表时间：
1997-02-14
期刊：
影响因子：
2.9
通讯作者：
Carafoli, E
中科院分区：
文献类型：
--
作者：
Stauffer, TP;Guerini, D;Carafoli, E
神经细胞中的Ca 2+稳态依赖于至少三种机制：Ca 2+通道、钙结合蛋白和Ca 2+交换器/泵。只有有限的信息可用于这些钙离子调节系统在大脑中的区域/细胞分布。在这项研究中，三个异构体的系统之一，质膜Ca 2 +-ATP酶（PMCA）的分布进行了分析。使用针对每种亚型的特异性表位的抗体，绘制了整个大脑中泵的分布图。该泵主要在神经元中表达，而在胶质细胞中明显缺失。亚型1是普遍存在的，并发生在不同的，但总是显着的，几乎所有的神经细胞中的浓度。亚型2在小脑浦肯野细胞中丰富，但在其他脑区较少集中。亚型3主要位于神经元以外的位置，例如，它在脉络丛中丰富。这三种亚型被发现以高度特征性的方式分布，表明神经细胞对细胞内钙稳态的保持有不同的要求。
Ca2+ homeostasis in nerve cells is dependent on at least three mechanisms: Ca2+ channels, calcium-binding proteins and Ca2+ exchangers/pumps. Only limited information is available on the regional/cellular distribution of these Ca2+-regulating systems in the brain. The distribution of three of the isoforms of one of the systems, plasma membrane Ca2+-ATPase (PMCA), was analyzed in this study. Using antibodies against epitopes specific for each isoform, a map of the distribution of the pump in the whole brain was produced. The pump was mainly expressed in neurons and was apparently absent from glia cells. Isoform 1 was ubiquitous and occurred in varying, but always significant, concentrations in almost all nerve cells. Isoform 2 was abundant in cerebellar Purkinje cells but less concentrated in other brain regions. Isoform 3 had a predominantly extra neuronal location, e.g. it was abundant in the choroid plexuses. The three isoforms were found to be distributed in a highly characteristic manner, suggesting that nerve cells have different requirements for the preservation of their intracellular calcium homeostasis.