Comprehensive analysis of localization of 78 solute carrier genes throughout the subsections of the rat gastrointestinal tract
Comprehensive analysis of localization of 78 solute carrier genes throughout the subsections of the rat gastrointestinal tract
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DOI：
10.1016/j.bbrc.2011.07.005
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发表时间：
2011-08-12
影响因子：
3.1
通讯作者：
Schioth, Helgi B.
中科院分区：
文献类型：
--
作者：
Cedernaes, Jonathan;Olszewski, Pawel K.;Schioth, Helgi B.
溶质载体（SoluteCarrier，SLC）是人类基因组中第二大的膜蛋白超家族，可跨膜转运氨基酸、糖、脂肪酸、无机离子、必需金属和药物等。到目前为止，还没有研究提供SLC定位沿着整个胃肠道的综合分析。本研究的目的是利用生物信息学和分子生物学方法对SLC基因在大鼠Cl束沿着的定位进行全面的、节段特异性的描述。在Unigene数据库中筛选肠组织中的大鼠SLC条目。使用qPCR，我们测量了Cl道中注释基因的表达，所述Cl道分为以下区段：食管、胃体和胃窦、十二指肠的近端和远端部分、回肠、空肠和结肠以及盲肠。我们的Unigene来源的基因库用来自内部组织面板和文献检索的数据进行了扩展。我们发现44的78（56%）的肠道SLC转录表达在所有的Cl道段，而大多数剩余的SLC中检测到超过五个部分。SLC主要在具有吸收功能的肠道区域表达，尽管在与吸收无关的节段中也发现了表达。近端空肠有最高数量的差异表达的SLC。总之，SLC是Cl道的关键分子组分，其中许多SLC沿沿着整个GI道表达。这项工作提出了第一个整体路线图的本地化转运蛋白基因在Cl道。(C)2011 Elsevier Inc. All rights reserved.
Solute carriers (SLCs), the second largest super-family of membrane proteins in the human genome, transport amino acids, sugars, fatty acids, inorganic ions, essential metals and drugs over membranes. To date no study has provided a comprehensive analysis of SLC localization along the entire GI tract. The aim of the present study was to provide a comprehensive, segment-specific description of the localization of SLC genes along the rat Cl tract by employing bioinformatics and molecular biology methods. The Unigene database was screened for rat SLC entries in the intestinal tissue. Using qPCR we measured expression of the annotated genes in the Cl tract divided into the following segments: the esophagus, the corpus and the antrum of the stomach, the proximal and distal parts of the duodenum, ileum, jejunum and colon, and the cecum. Our Unigene-derived gene pool was expanded with data from in-house tissue panels and a literature search. We found 44 out of 78 (56%) of gut SLC transcripts to be expressed in all Cl tract segments, whereas the majority of remaining SLCs were detected in more than five segments. SLCs are predominantly expressed in gut regions with absorptive functions although expression was also found in segments unrelated to absorption. The proximal jejunum had the highest number of differentially expressed SLCs. In conclusion, SLCs are a crucial molecular component of the Cl tract, with many of them expressed along the entire GI tract. This work presents the first overall road map of localization of transporter genes in the Cl tract. (C) 2011 Elsevier Inc. All rights reserved.