Kinetics of virus-specific CD8+-T-cell expansion and trafficking following central nervous system infection
Kinetics of virus-specific CD8+-T-cell expansion and trafficking following central nervous system infection
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DOI：
10.1128/jvi.77.4.2775-2778.2003
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发表时间：
2003-02-01
影响因子：
5.4
通讯作者：
Bergmann, CC
中科院分区：
文献类型：
--
作者：
Marten, NW;Stohlman, SA;Bergmann, CC
CD8+T细胞控制嗜神经性小鼠肝炎病毒对中枢神经系统（CNS）的急性感染，但不足以实现无菌免疫。为了确定中枢神经系统内T细胞启动和最佳活性之间的滞后，通过使用γ干扰素特异性ELISPOT测定和I类四聚体染色来评估中枢神经系统中病毒特异性CD8+T细胞相对于外周淋巴器官中的积累。首先在颈部淋巴结中检测到病毒特异性CD8(+) T细胞。脾脏的扩张被延迟且不太明显，但也先于中枢神经系统的积累。数据进一步表明外周细胞获得了溶细胞功能，从而增强了中枢神经系统中同源抗原识别后的CD8(+)-T细胞效应器功能。
CD8(+) T cells control acute infection of the central nervous system (CNS) by neurotropic mouse hepatitis virus but do not suffice to achieve sterile immunity. To determine the lag between T-cell priming and optimal activity within the CNS, the accumulation of virus-specific CD8(+) T cells in the CNS relative to that in peripheral lymphoid organs was assessed by using gamma interferon-specific ELISPOT assays and class I tetramer staining. Virus-specific CD8(+) T cells were first detected in the cervical lymph nodes. Expansion in the spleen was delayed and less pronounced but also preceded accumulation in the CNS. The data further suggest peripheral acquisition of cytolytic function, thus enhancing CD8(+)-T-cell effector function upon cognate antigen recognition in the CNS.