Mechanobiology of mandibular distraction osteogenesis: experimental analyses with a rat model
Mechanobiology of mandibular distraction osteogenesis: experimental analyses with a rat model
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DOI：
10.1016/j.bone.2003.10.012
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发表时间：
2004-02-01
期刊：
影响因子：
4.1
通讯作者：
Carter, DR
中科院分区：
文献类型：
--
作者：
Loboa, EG;Fang, TD;Carter, DR
我们分析了机械生物学对成功牵引成骨（DO）的影响。对 15 只成年雄性 Sprague-Dawley 大鼠进行下颌牵引手术。动物经历逐渐分散（GD），以小增量逐渐延长（5天潜伏期，然后每天两次0.25毫米分散，持续8天，然后是28天的成熟期）。在术后第 5、7、10、13 和 41 天（POD）收获分心的半下颌骨。然后记录 0.25 毫米离体分心的负载-位移曲线并确定应力。组织学上，在牵引第 2 天（POD 7）时，GD 标本中出现新骨形成，在牵引第 8 天（POD 13）时填充了 50-60% 的间隙，在成熟末期（POD 41）几乎完全形成骨桥。在牵引第 2-8 天期间，每次增量牵引施加的平均拉伸应变范围为约 10% 至 12.5%，并且与约 260 mum/天的骨附着率相关。由于该 GD 方案先前被确定为 DO 的最佳方案，因此我们得出结论，该范围内的菌株为从头骨接合提供了极好的环境。负载/位移曲线的明显下降证明，在分心第 2、5 和 8 天的样本中，分心导致了组织损伤。总而言之，我们对这些数据的解释是，日常分心会导致日常组织损伤，从而引发新的间充质组织形成。 (C) 2003 Elsevier Inc. 保留所有权利。
We analyzed mechanobiological influences on successful distraction osteogenesis (DO). Mandibular distraction surgeries were performed on 15 adult male Sprague-Dawley rats. Animals underwent gradual distraction (GD), progressive lengthening by small increments (5-day latency followed by 0.25 mm distractions twice daily for 8 days followed by 28-day maturation period). Distracted hemimandibles were harvested on postoperative days (POD) 5, 7, 10, 13, and 41. Load-displacement curves were then recorded for ex vivo distractions of 0.25 mm and stresses determined. Histologically, new bone formation appeared in GD specimens on distraction day 2 (POD 7), filling 50-60% of the gap by distraction day 8 (POD 13), with nearly complete bony bridging at end maturation (POD 41). Average tensile strains imposed by each incremental distraction ranged from approximately 10% to 12.5% during distraction days 2-8 and were associated with bone apposition rates of about 260 mum/day. Because this GD protocol was previously determined to be optimal for DO, we conclude that strains within this range provide an excellent environment for de novo bone apposition. Distraction caused tissue damage in distraction day 2, 5, and 8 specimens as evidenced by distinct drops in the load/displacement curves. Taken together, our interpretation of these data is that daily distractions cause daily tissue damage which triggers new mesenchymal tissue formation. (C) 2003 Elsevier Inc. All rights reserved.