Twist is required for patterning the cranial nerves and maintaining the viability of mesodermal cells
Twist is required for patterning the cranial nerves and maintaining the viability of mesodermal cells
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DOI：
10.1002/dvdy.20047
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发表时间：
2004-06
影响因子：
2.5
通讯作者：
M. Ota;D. Loebel;Meredith P O'Rourke;Nicole Wong;Bonny M Tsoi;P. Tam
中科院分区：
文献类型：
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作者：
M. Ota;D. Loebel;Meredith P O'Rourke;Nicole Wong;Bonny M Tsoi;P. Tam
Twist编码一个基本的螺旋-环-螺旋转录因子，这是小鼠正常颅面形态发生所必需的。颅间充质中Twist活性的丧失会导致神经脊细胞的异常迁移行为，而Twist缺陷的神经脊细胞位于第一鳃弓中的不适当位置，并显示出缺陷的成骨和成牙分化(Soo等人)。[2002]开发人员比奥尔。247：251-270)。本研究的结果进一步表明，扭转的丧失影响了脑神经节和神经的模式，但不影响躯干区域的外周神经节和神经的模式。对近轴中胚层早期分化的分子标记的表达和扭转/胚胎体节的组织发生能力的分析表明，扭转缺陷的体节可以分化成肌肉、软骨和骨骼，尽管分化的数量较少。因此，扭转函数对中胚层分化不是必需的。Twist缺陷的体节在移植到异位部位后生长不良，可能是由于旁中胚层的增殖能力降低和广泛的凋亡所致，提示Twist是维持中胚层前体细胞增殖和存活所必需的。《发展动力学》230：216-228,2004。©2004 Wiley-Liss Inc.
Twist encodes a basic helix‐loop‐helix transcription factor that is required for normal craniofacial morphogenesis in the mouse. Loss of Twist activity in the cranial mesenchyme leads to aberrant migratory behaviour of the neural crest cells, whereas Twist‐deficient neural crest cells are located in an inappropriate location in the first branchial arch and display defective osteogenic and odontogenic differentiation (Soo et al. [ 2002 ] Dev. Biol. 247:251–270). Results of the present study further show that loss of Twist impacts on the patterning of the cranial ganglia and nerves but not that of the peripheral ganglia and nerves in the trunk region of the body axis. Analyses of the expression of molecular markers of early differentiation of the paraxial mesoderm and the histogenetic potency of somites of Twist‐/‐ embryos reveal that Twist‐deficient somites can differentiate into muscles, cartilage, and bones, albeit less prolifically. Twist function, therefore, is not essential for mesoderm differentiation. The poor growth of the Twist‐deficient somites after transplantation to the ectopic site may be attributed to reduced proliferative capacity and extensive apoptosis of the paraxial mesoderm, suggesting that Twist is required for maintaining cell proliferation and viability in the mesodermal progenitors. Developmental Dynamics 230:216–228, 2004. © 2004 Wiley‐Liss, Inc.