Analysis of the in vitro adherence of Streptococcus mutans and Candida albicans
Analysis of the in vitro adherence of Streptococcus mutans and Candida albicans
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DOI：
10.1590/s1517-83822007000400009
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发表时间：
2007-12-01
影响因子：
2.2
通讯作者：
Pinheiro, Rosângela Lameira
中科院分区：
文献类型：
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作者：
Barbieri, Dicler de Sant'Anna Vitor;Vicente, Vânia Aparecida;Pinheiro, Rosângela Lameira
本研究的目的是研究变形链球菌和白色念珠菌的体外粘附能力。在牙齿表面进行粘附测定，并通过扫描电子显微镜 (SEM) 进行分析。拔出的人类牙齿同时接种变形链球菌或白色念珠菌，并在 37 摄氏度下培养 21 天。细菌接种物是从被这两种生物定植的儿童的唾液样本中获得的。 ATCC 参考菌株用作对照。 SEM 分析表明，覆盖整个分析牙齿表面的生物膜同时接种两种微生物比分别接种每种微生物更均匀。在第二个实验中，用表现出最高粘附力的分离株进行，测试分离株对高密度聚乙烯基材的粘附力。证实了变形链球菌和白色念珠菌联合时的粘附能力的潜力。
The objective of the present study was to investigate the in vitro adherence capacity of Streptococcus mutans and Candida albicans. Adherence assays were conducted on dental surfaces and analyzed by scanning electron microscopy (SEM). Extracted human teeth were inoculated with Streptococcus mutans or Candida albicans and with both species simultaneously, and incubated at 37 degrees C for 21 days. Bacterial inocula had been obtained from saliva samples of children that had been colonized by both organisms. ATCC reference strains were used as controls. SEM analyses showed that the biofilm that covered the entire analyzed dental surface was more homogeneous inoculated with the two microorganisms simultaneously than with each species separately. In a second experiment, carried out with isolates that had shown the highest adherence the isolates were tested for adherence to high-density polyethylene substrates. The potentialization of the adherence capacity of Streptococcus mutans and Candida albicans when in association was confirmed.