Characterization of antigens expressed in normal baboon trophoblast and cross-reactive with HIV/SIV antibodies
Characterization of antigens expressed in normal baboon trophoblast and cross-reactive with HIV/SIV antibodies
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DOI：
10.1016/s0165-0378(98)00043-6
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发表时间：
1999-01-01
影响因子：
3.4
通讯作者：
Mwenda, JM
中科院分区：
文献类型：
--
作者：
Langat, DK;Johnson, PM;Mwenda, JM
电子显微镜研究显示，正常灵长类胎盘组织中存在内源性逆转录病毒(ERV)颗粒。这些颗粒与C型逆转录病毒颗粒具有超微结构相似之处，主要与滋养层细胞有关。在正常人类胎盘组织中，它们与外源性逆转录病毒，如人类免疫缺陷病毒(HIV)具有抗原相似性，并可能在调节细胞基因表达、合体滋养层形成或妊娠相关免疫抑制中发挥作用。本研究用免疫组织化学和免疫印迹法检测了一组针对病毒蛋白(抗HIV和抗SIV)和内源性逆转录病毒(ERV)蛋白的抗体(多克隆和单抗)与从正常狒狒胎盘组织中分离的ERV颗粒的交叉反应。这些抗体(抗HERV-K RT、抗ERV-3 env、抗HIV-1 p17、抗HIV-2 gp120)与合体滋养层细胞呈阳性反应，每个抗体可识别一种或两种相对分子质量(Mw)38、58或kDa的蛋白质，存在于感染SIV的恒河猴胎盘绒毛组织和MOLT-II CL-8细胞中，而在未感染细胞中不识别。这一研究结果证实了逆转录病毒交叉反应抗原在正常狒狒胎盘组织中的特异性表达，并提示胎盘细胞蛋白可能与抗HIV/SIV抗体识别的蛋白具有抗原相似性。这些在母胎界面表达的逆转录病毒相关蛋白的作用尚不清楚。(C)1999爱思唯尔爱尔兰科学有限公司。保留所有权利。
Electron microscopic studies have revealed the presence of endogenous retroviral (ERV) particles in normal primate placental tissues. These particles have ultrastructural similarities to type C retroviral particles and are mainly associated with the trophoblast. In normal human placental tissues, they have antigenic similarity with exogenous retroviruses, such as the human immunodeficiency virus (HIV), and may have a role to play in the regulation of cellular gene expression, syncytiotrophoblast formation or pregnancy-related immunosuppression. In this study, a panel of antibodies (polyclonal and monoclonal antibodies) against viral proteins (anti-HIV and anti-SIV) and endogenous retroviral (ERV) proteins were assessed by immunohistochemistry and immunoblotting, for their cross-reactivity with ERV particles isolated from normal baboon placental tissues. The antibodies (anti-HERV-K RT, anti-ERV3 env, anti-HIV-1 p17, anti-HIV-2 gp120) reacted positively with the syncytiotrophoblast and each antibody recognized one or two proteins of molecular weights (MW) 38, 58 or 64 kDa present in the baboon placental villous tissues and SIV-infected molt-ii Cl-8 cells, but not in uninfected cells. The results of this study confirm the specific expression of retroviral cross-reactive antigens in normal baboon placental tissues and suggest placental cellular proteins may have antigenic similarity with those recognized by anti-HIV/SIV antibodies. The role of these retroviral-related proteins expressed at the maternal-fetal interface remain unclear. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.