Small RNA library cloning procedure for deep sequencing of specific endogenous siRNA classes in Caenorhabditis elegans.
Small RNA library cloning procedure for deep sequencing of specific endogenous siRNA classes in Caenorhabditis elegans.
复制标题
用于对秀丽隐杆线虫中特定内源 siRNA 类别进行深度测序的小 RNA 文库克隆程序。
DOI：
10.1007/978-1-4939-0931-5_6
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Hall,SarahE
中科院分区：
文献类型：
--
作者：
Ow,MariaC;Lau,NelsonC;Hall,SarahE
In recent years, distinct classes of small RNAs ranging in size from ~21 to 26 nucleotides have been discovered and shown to play important roles in a wide array of cellular functions. Because of the abundance of these small RNAs, library preparation from an RNA sample followed by deep sequencing provides the identity and quantity of a particular class of small RNAs. In this chapter we describe a detailed protocol for preparing small RNA libraries for deep sequencing on the Illumina platform from the nematodeC. elegans.
