Mapping and characterization of new EST-derived microsatellites for potato (Solanum tuberosum L.)
Mapping and characterization of new EST-derived microsatellites for potato (Solanum tuberosum L.)
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DOI：
10.1007/s00122-005-2028-2
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发表时间：
2005-08-01
影响因子：
5.4
通讯作者：
Lorenzen, J
中科院分区：
文献类型：
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作者：
Feingold, S;Lloyd, J;Lorenzen, J
微卫星或简单重复序列（SSR）是许多植物物种非常有用的分子标记。它们通常用于品种鉴定、植物品种保护、作为遗传作图中的锚标记以及标记辅助育种。SSR的早期开发受到文库筛选和克隆测序的高成本的阻碍。目前，许多作物物种都有大型的公共SSR数据集，但马铃薯的公共SSR图谱数量相对较低（接近100个）。我们已经利用数据库挖掘方法来鉴定基因组研究所马铃薯基因索引数据库（http：//www.example.com）中的含SSR的序列，集中于该数据集中存在的具有大小多态性的序列。www.tigr.org合成了94对SSR引物，用于扩增马铃薯DNA。这项研究提供了61个有用的SSR，这些SSR位于预先存在的遗传图谱中，在来自南美，北美和欧洲或其组合的30个品种中指纹图谱。在这项工作中获得的表达序列标签衍生的SSR的高成功率（65%）验证了转录序列作为标记物来源的用途。这些标记将有助于遗传作图、分类学研究、标记辅助选择和品种鉴定。
Microsatellites, or simple sequence repeats (SSRs) are very useful molecular markers for a number of plant species. They are commonly used in cultivar identification, plant variety protection, as anchor markers in genetic mapping, and in marker-assisted breeding. Early development of SSRs was hampered by the high cost of library screening and clone sequencing. Currently, large public SSR datasets exist for many crop species, but the number of publicly available, mapped SSRs for potato is relatively low (similar to 100). We have utilized a database mining approach to identify SSR-containing sequences in The Institute For Genomic Research Potato Gene Index database (http://www.tigr.org), focusing on sequences with size polymorphisms present in this dataset. Ninety-four primer pairs flanking SSR sequences were synthesized and used to amplify potato DNA. This study rendered 61 useful SSRs that were located in pre-existing genetic maps, fingerprinted in a set of 30 cultivars from South America, North America, and Europe or a combination thereof. The high proportion of success (65%) of expressed sequence tag-derived SSRs obtained in this work validates the use of transcribed sequences as a source of markers. These markers will be useful for genetic mapping, taxonomic studies, marker-assisted selection, and cultivar identification.