Adaptation to low temperatures in the wild tomato species Solanum chilense
Adaptation to low temperatures in the wild tomato species Solanum chilense
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DOI：
10.1111/mec.13637
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发表时间：
2016-06
影响因子：
4.9
通讯作者：
T. Nosenko;Katharina B. Böndel;Gabriele Kumpfmüller;W. Stephan
中科院分区：
文献类型：
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作者：
T. Nosenko;Katharina B. Böndel;Gabriele Kumpfmüller;W. Stephan
植物对非生物胁迫的分子适应是一个复杂的过程，主要基于基因转录活性的改变和蛋白质-蛋白质相互作用的改变。我们采用种群遗传学、比较转录学和植物生理学相结合的方法来研究沿安第斯海拔梯度分布的辣椒茄子种群对低温的适应机制。我们发现，所有种群的植物都具有很高的耐冷性，这与它们原生栖息地的温度无关。相比之下，对冰冻的耐受性与海拔和温度变量有显著的关联。我们还观察了来自高海拔和低海拔种群的植物之间冷反应基因表达模式的差异。这些结果表明，对低温的遗传适应是在高海拔地区的辣椒种群中进化出来的。在转录水平上，这些适应可能包括编码ICE1和叶绿体ω-3脂肪酸去饱和酶FAD7的基因的高水平结构性表达。ICE1是冷信号通路的关键转录因子。在序列水平上，在编码ACT调节域的ICE1的C-末端部分检测到与高海拔适应相关的选择特征。
Molecular adaptation to abiotic stresses in plants is a complex process based mainly on the modifications of gene transcriptional activity and the alteration of protein–protein interactions. We used a combination of population genetic, comparative transcriptomic and plant physiology approaches to investigate the mechanisms of adaptation to low temperatures in Solanum chilense populations distributed along Andean altitudinal gradients. We found that plants from all populations have high chilling tolerance, which does not correlate with temperatures in their native habitats. In contrast, tolerance to freezing shows a significant association with altitude and temperature variables. We also observed the differences in expression patterns of cold‐response genes between plants from high‐ and low‐altitude populations. These results suggest that genetic adaptations to low temperatures evolved in high‐altitude populations of S. chilense. At the transcriptional level, these adaptations may include high levels of constitutive expression of the genes encoding ICE1, the key transcription factor of the cold signalling pathway, and chloroplast ω‐3 fatty acid desaturase FAD7. At the sequence level, a signature of selection associated with the adaptation to high altitudes was detected at the C‐terminal part of ICE1 encoding the ACT regulatory domain.