Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons.
Peripheral nerve fibroblasts secrete neurotrophic factors to promote axon growth of motoneurons.
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周围神经成纤维细胞分泌神经营养因子促进运动神经元轴突生长
DOI：
10.4103/1673-5374.332159
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发表时间：
2022-08
影响因子：
6.1
通讯作者：
Ding F
中科院分区：
文献类型：
--
作者：
He QR;Cong M;Yu FH;Ji YH;Yu S;Shi HY;Ding F
周围神经成纤维细胞在神经发育和再生中起关键作用。我们前期的研究发现周围神经成纤维细胞具有不同的感觉和运动表型。不同表型的成纤维细胞可以引导雪旺细胞迁移到相同的感觉或运动表型。本研究分析了外周神经源性成纤维细胞和心脏成纤维细胞对运动神经元的不同影响。与心脏成纤维细胞相比，周围神经成纤维细胞显著促进运动神经元突起的生长。转录组分析结果确定了491个基因在外周神经成纤维细胞和心脏成纤维细胞中差异表达。其中，130个在外周神经成纤维细胞中与心脏成纤维细胞相比显著上调。这些基因可能参与轴突导向和神经元投射。大鼠坐骨神经切断后3天，周围神经成纤维细胞在近端和远端神经残端聚集，并大多表达脑源性神经营养因子。在体外，周围神经成纤维细胞分泌的脑源性神经营养因子通过细胞外调节蛋白激酶和丝氨酸/苏氨酸激酶途径增加β-actin和F-actin的表达，促进运动神经元突起生长。这些发现表明，外周神经成纤维细胞和心脏成纤维细胞表现出不同的基因表达模式。周围神经成纤维细胞能促进运动神经元突起的生长。
Peripheral nerve fibroblasts play a critical role in nerve development and regeneration. Our previous study found that peripheral nerve fibroblasts have different sensory and motor phenotypes. Fibroblasts of different phenotypes can guide the migration of Schwann cells to the same sensory or motor phenotype. In this study, we analyzed the different effects of peripheral nerve-derived fibroblasts and cardiac fibroblasts on motoneurons. Compared with cardiac fibroblasts, peripheral nerve fibroblasts greatly promoted motoneuron neurite outgrowth. Transcriptome analysis results identified 491 genes that were differentially expressed in peripheral nerve fibroblasts and cardiac fibroblasts. Among these, 130 were significantly upregulated in peripheral nerve fibroblasts compared with cardiac fibroblasts. These genes may be involved in axon guidance and neuron projection. Three days after sciatic nerve transection in rats, peripheral nerve fibroblasts accumulated in the proximal and distal nerve stumps, and most expressed brain-derived neurotrophic factor. In vitro, brain-derived neurotrophic factor secreted from peripheral nerve fibroblasts increased the expression of β-actin and F-actin through the extracellular regulated protein kinase and serine/threonine kinase pathways, and enhanced motoneuron neurite outgrowth. These findings suggest that peripheral nerve fibroblasts and cardiac fibroblasts exhibit different patterns of gene expression. Peripheral nerve fibroblasts can promote motoneuron neurite outgrowth.
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影响因子：
4.8
作者：
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通讯作者：
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影响因子：
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作者：
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