The effect of LH on the fertilizability and developmental capacity of rat oocytes matured in vitro.
The effect of LH on the fertilizability and developmental capacity of rat oocytes matured in vitro.
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LH对体外成熟大鼠卵母细胞受精能力和发育能力的影响。
DOI：
10.1530/jrf.0.0550429
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
P. Kraicer
中科院分区：
文献类型：
--
作者：
R. Shalgi;N. Dekel;P. Kraicer
研究了在卵母细胞体外成熟的培养基中添加LH（10微克NIH-LH-B8/ml）的效果。在单侧卵巢切除的交配受体的无菌输卵管中评估卵母细胞的受精能力。新鲜排卵的卵母细胞用作该方法的对照，受精率为 72%。培养成熟的卵母细胞（不含 LH）中只有 14% 被精子穿透，11% 正常受精。向培养基中添加 LH 使这些比例分别增加至 43% 和 33%。在 LH 存在下成熟的卵母细胞能够发育成表面上正常的大鼠。结论是，虽然卵母细胞可以在体外自发成熟，并且这些成熟的卵母细胞可以受精，但添加LH使数量增加3倍。因此，LH 在体外对大鼠卵母细胞-卵丘复合体具有直接的成熟促进作用。
The effect of adding LH (10 microgram NIH-LH-B8/ml) to the medium in which oocytes were undergoing maturation in vitro was studied. The fertilizability of the oocytes was evaluated in the sterile oviduct of a unilaterally ovariectomized, mated recipient. Freshly ovulated oocytes, used as a control of the method, were fertilized at a rate of 72%. Only 14% of oocytes matured in culture (without LH) were penetrated by spermatozoa, and 11% were fertilized normally. Addition of LH to the medium increased these proportions to 43 and 33% respectively. Oocytes matured in the presence of LH were able to develop into apparently normal rats. It is concluded that, although oocytes can mature in vitro spontaneously, and that these matured oocytes can be fertilized, addition of LH increases the numbers 3-fold. LH therefore has a direct maturation-promoting action on the rat oocyte-cumulus complex in vitro.