Proteomic identification of differentially-expressed proteins in squamous cervical cancer.
Proteomic identification of differentially-expressed proteins in squamous cervical cancer.
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DOI：
10.1016/j.ygyno.2008.09.045
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发表时间：
2009
影响因子：
4.7
通讯作者：
Xueqiong Zhu;J. Lv;Li-rong Yu;Xuejie Zhu;Jie-li Wu;Shuangwei Zou;Shanshan Jiang
中科院分区：
文献类型：
--
作者：
Xueqiong Zhu;J. Lv;Li-rong Yu;Xuejie Zhu;Jie-li Wu;Shuangwei Zou;Shanshan Jiang
目的通过蛋白质组学方法鉴定宫颈癌的候选生物标志物，并揭示该疾病的分子机制。方法采用双向凝胶电泳法分离10对人宫颈鳞癌和与其相匹配的宫颈正常组织的蛋白，采用基质辅助激光解吸/电离飞行时间质谱法鉴定差异表达蛋白。然后，通过Western blotting在另外20对组织中证实了获得的一些感兴趣的蛋白质。结果与宫颈邻近正常组织相比，宫颈鳞癌组织中55个蛋白斑发生显著变化，其中24个蛋白斑强度一致升高，31个蛋白斑强度一致降低。其中32种蛋白通过质谱鉴定。免疫印迹法证实了Tyk2、S100A9和锌指蛋白217在宫颈鳞癌中的过表达。结论基于蛋白质组学的方法有助于更全面地了解宫颈鳞状癌的蛋白质谱。对这些差异蛋白的进一步分析将确定其在宫颈鳞癌特异性诊断和治疗中的潜在适用性。
OBJECTIVETo identify candidate biomarkers for squamous cervical cancer as well as reveal the molecular mechanism underlying this disease by a proteomic approach.METHODSProteins from 10 pairs of human squamous cervical cancer and matching adjacent normal cervical tissues were separated by two-dimensional gel electrophoresis and the differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Then, some of the interesting proteins obtained were confirmed by Western blotting in the other 20 pairs of tissues.RESULTSA comparison of protein patterns revealed 55 protein spots significantly changed, of which 24 protein spots with concordantly increased and 31 protein spots with concordantly decreased intensity in squamous cervical cancer compared with adjacent normal cervical tissues. Thirty-two of these proteins were identified by mass spectrometry. The overexpression of the Tyk2, S100A9, and Zinc finger protein 217 in squamous cervical cancer was confirmed by immunoblotting.CONCLUSIONSOur study suggested that a proteomics-based approach is useful for developing a more complete picture of the protein profile of squamous cervical cancer. Further ongoing analysis of these differential proteins will determine their potential applicability to squamous cervical cancer-specific diagnosis and therapeutics.