Induction of astrocyte argininosuccinate synthetase and argininosuccinate lyase by dibutyryl cyclic AMP and dexamethasone.
Induction of astrocyte argininosuccinate synthetase and argininosuccinate lyase by dibutyryl cyclic AMP and dexamethasone.
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二丁酰环 AMP 和地塞米松诱导星形胶质细胞精氨琥珀酸合成酶和精氨琥珀酸裂合酶。
DOI：
10.1007/bf02532390
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发表时间：
1996
影响因子：
4.4
通讯作者：
Zielke,CL
中科院分区：
文献类型：
--
作者：
Jackson,MJ;Zielke,HR;Zielke,CL
精氨酸是消除过量氮的中间产物，也是合成一氧化氮的底物。据报道，精氨酸在脑组织中合成。我们研究了地塞米松和/或二丁酰环AMP处理的大鼠星形胶质细胞培养过程中精氨酸生物合成酶、精氨酸琥珀酸合成酶和精氨酸琥珀酸裂解酶的活性。精氨酸琥珀酸裂解酶活性可被两种效应剂中的任何一种处理所刺激，当两种效应剂同时加入时，可获得相加效应。地塞米松处理的星形胶质细胞精氨酸琥珀酸合成酶也升高。二丁酰环AMP对精氨酸琥珀酸合成酶的影响是不同的，这表明与精氨酸琥珀酸裂解酶相比，在精氨酸琥珀酸合成酶的调节中有其他因素的作用。调节星形胶质细胞的精氨酸合成可能是重要的，以确保精氨酸不会限制神经组织中一氧化氮的合成。
Arginine is an intermediate in the elimination of excess nitrogen and is the substrate for nitric oxide synthesis. Arginine synthesis has been reported in brain tissue. We have studied the activity of the arginine biosynthetic enzymes argininosuccinate synthetase and argininosuccinate lyase in dexamethasone and/or dibutyryl cyclic AMP treated rat astrocyte cultures. Argininosuccinate lyase activity was stimulated by treatment with either effector and an additive effect was obtained when both agents were added simultaneously. Argininosuccinate synthetase was also increased in dexamethasone treated astrocytes. The effect of dibutyryl cyclic AMP on argininosuccinate synthetase was variable, suggesting a role for additional factors in its regulation as compared to argininosuccinate lyase. Regulation of arginine synthesis in astrocytes may be important to insure that arginine is not limiting for nitric oxide synthesis in neural tissue.