Long-term monitoring of pulmonary arterial pressure in conscious, unrestrained mice
Long-term monitoring of pulmonary arterial pressure in conscious, unrestrained mice
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DOI：
10.1016/j.vascn.2005.11.003
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发表时间：
2006-05-01
影响因子：
1.9
通讯作者：
Shirai, Miklyasu
中科院分区：
文献类型：
--
作者：
Schwenke, Daryl O.;Pearson, James T.;Shirai, Miklyasu
简介：基因工程特异性基因“敲除”小鼠的能力为研究导致肺动脉高压（PAH）发病的各种机制提供了强有力的工具。然而，到目前为止，还没有报道描述的肺动脉压（PAP）的测量在清醒的野生型小鼠的监测动态变化与PAH的发病机制的基本要求。因此，在这项研究中，我们描述了一种新的技术，长期测量PAP在清醒的无约束小鼠使用遥测。方法：在5只雄性C57 BL/6小鼠（B.W. 25-30 g）时，将遥测发射器的传感导管插入右心室并推进到肺动脉中。发送器主体位于腹腔内或背部皮下。在手术恢复期间，每天记录平均PAP，持续1周。随后，PAP对急性缺氧（8%O-2，10 min）和L-NAME（50 mg/kg，s.c.）在三只老鼠身上测试。结果：术后1周。所有的小鼠都从手术中完全恢复，并且基线MPAP稳定在14.9 +/-0.7分钟汞柱。此外，肺血管兴奋剂急性缺氧和L-NAME引起IMPAP分别增加63%和86%。讨论：总之，本研究证明了通过遥测准确测量清醒、无约束小鼠PAP的能力。该技术在未来研究中的一个重要应用是评估肺病理条件慢性发展期间特定基因（使用“敲除”小鼠）的相对贡献的可能性。(c)2005年爱思唯尔公司All rights reserved.
Introduction: The ability to genetically engineer specific gene 'knock-out' mice has provided a powerful tool for investigating the various mechanisms that contribute to the pathogenesis of pulmonary arterial hypertension (PAH). Yet, so far there have been no reports describing the measurement of pulmonary arterial pressure (PAP) in the conscious wild type mouse-an essential requirement for monitoring dynamic changes associated with the pathogenesis of PAH. Therefore, in this study we describe a new technique for long-term measurement of PAP in conscious unrestrained mice using telemetry. Methods: In five male C57BL/6 mice (B.W. 25-30 g), the sensing catheter of a telemetric transmitter was inserted into the right ventricle and advanced into the pulmonary artery. The transmitter body was positioned either within the abdominal cavity or subcutaneously on the back. During recovery from surgery, mean PAP was recorded daily for I week. Subsequently, the PAP responses to acute hypoxia (8% O-2 for 10 min) and L-NAME (50 mg/kg, s.c.) were tested in three mice. Results: By 1-week post surgery. all mice had fully recovered from surgery and baseline MPAP was stable at 14.9 +/- 0.7 min Hg. Additionally, the pulmonary vascular stimulants acute hypoxia and L-NAME provoked a 63% and 86% increase IMPAP, respectively. Discussion: In summary, this study has demonstrated the ability to accurately measure PAP by telemetry in conscious, unrestrained mice. One important application of this technique for future studies is the possibility to assess the relative contribution of specific genes (using 'knock-out' mice) during the chronic development of pulmonary pathological conditions. (c) 2005 Elsevier Inc. All rights reserved.