The regulated expression of a diverse set of genes during thymocyte positive selection in vivo
The regulated expression of a diverse set of genes during thymocyte positive selection in vivo
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DOI：
10.4049/jimmunol.173.9.5434
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发表时间：
2004-11-01
影响因子：
4.4
通讯作者：
Hogquist, KA
中科院分区：
文献类型：
--
作者：
Mick, VE;Starr, TK;Hogquist, KA
A signal initiated by the newly formed Ag receptor is integrated with microenvironmental cues during T cell development to ensure positive selection of CD4(+)CD8(+) progenitors into functionally mature CD4(+) or CD8(+) T lymphocytes. During this transition, a survival program is initiated, TCR gene recombination ceases, cells migrate into a new thymic microenvironment, the responsiveness of the Ag receptor is tuned, and the cells commit to a specific T lineage. To determine potential regulators of these processes, we used mRNA microarray analysis to compare gene expression changes in CD4(+)CD8(+) thymocytes from TCR transgenic mice that have received a TCR selection signal with those that had not received a signal. We found 129 genes with expression that changed significantly during positive selection, the majority of which were not previously appreciated. A large number of these changes were confirmed by real-time PCR or How cytometry. We have combined our findings with gene changes reported in the literature to provide a comprehensive report of the genes regulated during positive selection, and we attempted to assign these genes to positive selection process categories.
