A flow cytometric study of 137 fresh hydropic placentas: correlation between types of hydatidiform moles and nuclear DNA ploidy.
A flow cytometric study of 137 fresh hydropic placentas: correlation between types of hydatidiform moles and nuclear DNA ploidy.
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137 个新鲜水肿胎盘的流式细胞术研究：葡萄胎类型与核 DNA 倍体之间的相关性。
DOI：
10.1097/00006250-199203000-00016
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发表时间：
1992
影响因子：
7.2
通讯作者：
Berkowitz,RS
中科院分区：
文献类型：
--
作者：
Lage,JM;Mark,SD;Roberts,DJ;Goldstein,DP;Bernstein,MR;Berkowitz,RS
根据组织病理学，积水胎盘可分为积水流产、部分性葡萄胎和完全性葡萄胎。我们研究了142个积水胎盘：39%为完全性葡萄胎，35%为部分性葡萄胎，26%为积水流产。绒毛泡大小是组织学诊断的预测指标。我们对137例进行了DNA倍体检测。水中流产中73%为二倍体，11%为三倍体。90%的部分葡萄胎为三倍体或近三倍体，1例为单倍体，1例为二倍体。在完全葡萄胎中，二倍体占50%，四倍体占43%，多倍体占3.6%，三倍体占1.7%。部分葡萄胎排出前[β]-hCG水平低于完全性葡萄胎。33%的完全性葡萄胎和12%的部分性葡萄胎伴发持续性肿瘤。虽然数量很少，但二倍体、四倍体、非整倍体或单倍体部分葡萄胎的患者没有发生持续性疾病。在完全性葡萄胎中，排空前[β]-hCG水平不能预测持续性(P=.15)。通过对完全胎盘进行倍性细分，我们发现四倍体胎盘与排空前[β]-hCG水平高于二倍体胎盘。然而，四倍体与完全性葡萄胎中持续性肿瘤的增加无关。虽然大多数部分葡萄胎是三倍体，大部分完全性葡萄胎是二倍体或四倍体，但在不同的葡萄胎妊娠中，DNA的异质性比以前报道的更广泛。在这个系列中，DNA倍体不是完全性葡萄胎持续存在的独立预测因素。
: Hydropic placentas may be classified by histopathology into hydropic abortus, partial hydatidiform mole, and complete hydatidiform mole. We studied 142 hydropic placentas: 39% were complete hydatidiform moles, 35% partial hydatidiform moles, and 26% hydropic abortuses. Villous vesicle size was predictive of histologic diagnosis. We determined DNA ploidy in 137 cases. Seventy-three percent of hydropic abortuses were diploid and 11% were triploid. Ninety percent of partial moles were triploid or near-triploid; one partial mole was haploid and one diploid. Of the complete moles, 50% were diploid, 43% were tetraploid, 3.6% polyploid, and 1.7% triploid. Partial moles had lower preevacuation [beta]-hCG levels than complete moles. Persistent tumor followed 33% of complete moles and 12% of partial moles. Although the numbers were small, no patient with a diploid, tetraploid, aneuploid, or haploid partial mole developed persistent disease. Among complete moles, the pre-evacuation [beta]-hCG level was not predictive of persistence (P=. 15). Subdividing complete moles by ploidy, we found that tetraploid moles were associated with higher pre-evacuation [beta]-hCG levels than were diploid moles. However, tetraploidy was not associated with increased persistent tumor among complete moles. Although most partial moles were triploid and most complete moles were diploid or tetraploid, there was wider DNA heterogeneity among molar gestations than previously reported. In this series, DNA ploidy was not an independent predictor of persistence in complete moles.(Obstet Gynecol 1992; 79: 403-10)