A Carbonized Fluorescent Nucleolus Probe Discloses RNA Reduction in the Process of Mitophagy
A Carbonized Fluorescent Nucleolus Probe Discloses RNA Reduction in the Process of Mitophagy
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DOI：
10.31635/ccschem.021.202101371
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发表时间：
2022-08-01
期刊：
影响因子：
11.2
通讯作者：
Xiao, Lehui
中科院分区：
文献类型：
--
作者：
Liu, Hua;Geng, Xin;Xiao, Lehui
线粒体自噬是一个复杂的细胞代谢过程，表现为多个细胞器之间动态的时空协调。尽管它与核仁相关，但由于难以特异性标记RNA，因此可视化核仁RNA在线粒体自噬过程中的分布仍然是一个巨大的挑战。在此，我们展示了一个强大的荧光探针与叶酸（FA）和间苯二胺（m-PD）作为前体，它显示上级RNA锚定能力和快速的细胞渗透性，以标记活细胞中的核仁RNA。更重要的是，该探针在生理条件下具有自发闪烁行为，适合于核仁RNA分布细微变化的纳米成像。总体而言，这项工作提出了一种新的方法，在活体细胞中的核仁RNA的纳米级行为的原位观察，提供了一个强大的方法，为进一步探索细胞代谢的线粒体自噬。[图形]。
Mitophagy is a complicated process of cell metabo-lism that exhibits dynamic spatiotemporal coordi-nation between multiple organelles. Despite its relevance to nucleoli, visualizing the distribution of nucleolar RNA in the process of mitophagy remains a great challenge because of the difficulty in specif-ically labeling RNA. Herein, we demonstrate a robust carbonized fluorescent probe with folic acid (FA) and m-phenylenediamine (m-PD) as the precursors, which displays superior RNA-anchoring ability and fast cellular permeability to tag nucleolar RNA in living cells. More importantly, this probe possesses spontaneous blinking behavior in physiological con-ditions, which is suitable for nanoscopic imaging of subtle changes in the distribution of nucleolar RNA. Overall, this work presents a new way for in situ observation of nanoscopic behavior of nucleolar RNA in living cells, providing a powerful approach for further exploration of cellular metabolism in mitophagy.[GRAPHICS].