Gongronella sp. w5 elevates Coprinopsis cinerea laccase production by carbon source syntrophism and secondary metabolite induction
Gongronella sp. w5 elevates Coprinopsis cinerea laccase production by carbon source syntrophism and secondary metabolite induction
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贡罗氏菌属
DOI：
10.1007/s00253-018-9469-4
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发表时间：
2019-01-01
影响因子：
5
通讯作者：
Fang, Zemin
中科院分区：
文献类型：
--
作者：
Hu, Jun;Zhang, Yinliang;Fang, Zemin
当将蔗糖用作碳源时，coprinopsis cinerea在纯培养中表现出较差的生长和低漆酶活性，但在粘膜培养过程中，粘膜活性水平（> 1800 u/l）与粘膜型肌培养物的水平（> 1800 U/L）。 W5。结果，通过阐明蔗糖的功能，研究了漆酶过量生产的机理。结果表明Gongronella sp。共培养系统中的W5水解蔗糖，葡萄糖和果糖果糖是细胞内转化酶。提供果糖而不是葡萄糖的ggongronella sp。 W5是灰叶梭菌的易于使用的碳源，并导致其生长行为的改变和基底漆酶分泌110.6 +/- 3.3u/l。另一方面，分开贡隆氏菌Sp。通过转移到透析管中的灰曲霉的W5产生的漆酶活性与没有分离的水平相同，这表明增强的漆酶产生可能是由于发酵汤中的代谢产生的。进一步的研究表明，乙酸乙酯提取的代谢产物由Gongronella sp产生。 W5诱导C. c. cinerea漆酶生产。纯化的p-羟基苯甲酸（HBA）诱导了漆酶的一种化合物之一，并从提取物中鉴定出来。当将HBA用作诱导剂和果糖作为单一培养中的碳源时，C. cinerea观察到与共培养中相似的高漆酶活性，并且Zymogrampons揭示了漆酶LCC9与Main和LCC1和LCC5与次要酶相同的表达。总体而言，我们的实验证实了Gongronella sp。 W5通过碳源综合症和继发代谢产物诱导来提升coprinoppis灰灰卷漆酶的产生。
When sucrose was used as the carbon source, the Basidiomycete Coprinopsis cinerea showed poor growth and low laccase activity in pure culture, but greatly enhanced the level of laccase activity (>1800 U/L) during coculture with the Mucoromycete Gongronella sp. w5. As a result, the mechanism of laccase overproduction in coculture was investigated by starting from clarifying the function of sucrose. Results demonstrated that Gongronella sp. w5 in the coculture system hydrolyzed sucrose to glucose and fructose by an intracellular invertase. Fructose rather than glucose was supplied by Gongronella sp. w5 as the readily available carbon source for C. cinerea, and contributed to an alteration of its growth behavior and a basal laccase secretion of 110.6 ± 3.3 U/L. On the other hand, separating Gongronella sp. w5 of C. cinerea by transfer into dialysis tubes yielded the same level of laccase activity as without separation, indicating that enhanced laccase production probably resulted from the metabolites in the fermentation broth. Further investigation showed that the ethyl acetate-extracted metabolites generated by Gongronella sp. w5 induced C. cinerea laccase production. One of the laccase-inducing compounds namely p-hydroxybenzoic acid (HBA) was purified and identified from the extract. When using HBA as the inducer and fructose as the carbon source in monoculture, C. cinerea observed similar high laccase activity to that in coculture, and zymograms revealed the same expression of laccase Lcc9 as the main and Lcc1 and Lcc5 as the minor enzymes. Overall, our experiments verified that Gongronella sp. w5 elevates Coprinopsis cinerea laccase production by carbon source syntrophism and secondary metabolite induction.