Biocompatibility evaluation of sputtered zirconium-based thin film metallic glass-coated steels.
Biocompatibility evaluation of sputtered zirconium-based thin film metallic glass-coated steels.
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DOI：
10.2147/ijn.s79977
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发表时间：
2015
影响因子：
8
通讯作者：
Rajan ST
中科院分区：
文献类型：
--
作者：
Subramanian B;Maruthamuthu S;Rajan ST
由Zr 48 Cu 36 Al 8Ag 8（at.%）组成的薄膜金属玻璃采用磁控溅射法在医用316L不锈钢上制备了厚度分别为1.5 μm和3 μm的两种不同厚度的薄膜。分析了它们的结构和机械性能、体外腐蚀性和抗菌活性。非晶薄膜金属玻璃由单一玻璃相组成，没有任何对应于结晶相的可检测峰。从薄膜的EDAX分析中注意到接近目标合金的元素组成。扫描电子显微镜和原子力显微镜观察到薄膜表面光滑。体外电化学腐蚀研究表明，锆基金属玻璃可以承受体液，表现出上级的耐腐蚀性和电化学稳定性。通过琼脂扩散法、溶液悬浮法和湿界面接触法研究了涂层表面与细菌之间的相互作用。结果表明，对微生物如大肠杆菌和金黄色葡萄球菌的生长有明显的抑制区，证实了薄膜金属玻璃的抗微生物活性。使用L929成纤维细胞的细胞毒性研究表明，这些涂层本质上无细胞毒性。
Thin film metallic glasses comprised of Zr48Cu36Al8Ag8 (at.%) of approximately 1.5 μm and 3 μm in thickness were prepared using magnetron sputtering onto medical grade 316L stainless steel. Their structural and mechanical properties, in vitro corrosion, and antimicrobial activity were analyzed. The amorphous thin film metallic glasses consisted of a single glassy phase, with an absence of any detectable peaks corresponding to crystalline phases. Elemental composition close to the target alloy was noted from EDAX analysis of the thin film. The surface morphology of the film showed a smooth surface on scanning electron microscopy and atomic force microscopy. In vitro electrochemical corrosion studies indicated that the zirconium-based metallic glass could withstand body fluid, showing superior resistance to corrosion and electrochemical stability. Interactions between the coated surface and bacteria were investigated by agar diffusion, solution suspension, and wet interfacial contact methods. The results indicated a clear zone of inhibition against the growth of microorganisms such as Escherichia coli and Staphylococcus aureus, confirming the antimicrobial activity of the thin film metallic glasses. Cytotoxicity studies using L929 fibroblast cells showed these coatings to be noncytotoxic in nature.