Comparison of cellular functionality of human mesenchymal stromal cells and PBMC
Comparison of cellular functionality of human mesenchymal stromal cells and PBMC
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DOI：
10.1080/14653240601011557
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发表时间：
2007-01-01
期刊：
影响因子：
4.5
通讯作者：
Mehhorn, A. T.
中科院分区：
文献类型：
--
作者：
Schmal, H.;Niemeyer, P.;Mehhorn, A. T.
背景人骨髓间充质干细胞（MSC）和外周血单个核细胞（PBMC）在炎症后的修复过程中发挥重要作用。方法和结果MIP-1 α诱导MSC趋化性迁移，但不诱导PBMC趋化性迁移。与此相关，7.7%的MSC表达趋化因子受体CCR-1，如FACS分析所示。相反，PBMC不表达CCR-1或CCR-2，但表达CXCR-4（81.9%）和CCR-7（42.2%）。血清诱导两种细胞类型的趋化性，酵母多糖活化增加PBMC的迁移，但不MSC。与此相对应，C5 a诱导PBMC而不是MSC的迁移。MSC与纤连蛋白、纤维蛋白原、I型胶原蛋白和H型胶原蛋白的粘附呈剂量依赖性和特异性;相反，PBMC不粘附任何研究的蛋白质。受体表达的实时PCR显示，与PBMC相比，MSC中C5 a-受体的表达高72.2倍，PBMC中C5 a-受体的表达高4.7倍。MSC与肿瘤坏死因子-α（TNF-α）共同孵育可诱导NA-κ B活化，并增加MSC对血小板的趋化反应和对纤维连接蛋白的粘附。
Background Human mesenchymal stromal cells (MSC) and PBMC play significant roles in repair processes following inflammation. Mechanisms of recruitment are still under investigation.Methods and results MIP-1 alpha induced the chemotactic migration of MSC but not of PBMC. Correlating with this, 7.7% of MSC expressed the chemokine receptor CCR-1, as shown by FACS analysis. In contrast, PBMC did not express CCR-1 or CCR-2 but did express CXCR-4 (81.9%) and CCR-7 (42.2%). Serum induced the chemotaxis of both cell types, and zymosan activation increased the migration of PBMC but not of MSC. Corresponding with this, C5a induced the migration of PBMC but not of MSC. Dose-dependent and -specific adhesion to fibronectin, fibrinogen, collagen type I and collagen type H could be demonstrated for MSC; in contrast, PBMC did not adhere to any of the investigated proteins. Real-time PCR of receptor expression revealed a 72.2-fold higher expression of)(v in MSC compared with PBMC, and a 4.7-fold higher expression of C5a-receptor in PBMC. Incubation of MSC with tumor necrosis factor-alpha (TNF alpha) induced NA kappa B activation and increased the chemotactic rerponse to scrum and adhesion to fibronectin.Discussion Chemotaxis and adhesion are crucial and differing cell functions of MSC and PBMC.