Evaluation of the effect of incorporation of dibutyryl cyclic adenosine monophosphate in an in situ-forming hydrogel wound dressing based on oxidized alginate and gelatin
Evaluation of the effect of incorporation of dibutyryl cyclic adenosine monophosphate in an in situ-forming hydrogel wound dressing based on oxidized alginate and gelatin
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DOI：
10.1016/j.biomaterials.2005.08.021
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发表时间：
2006-03-01
期刊：
影响因子：
14
通讯作者：
Jayakrishnan, A
中科院分区：
文献类型：
--
作者：
Balakrishnan, B;Mohanty, M;Jayakrishnan, A
环腺苷一磷酸（cAMP）一直被认为是人类角化细胞增殖的第二信使和调节剂。为了探索更有效的伤口管理，将cAMP的亲脂性类似物二丁基环腺苷单磷酸（DBcAMP）掺入基于高碘酸盐氧化海藻酸盐和明胶的原位形成水凝胶伤口敷料中。DBcAMP从基质向磷酸盐缓冲盐水的体外释放缓慢且随时间增加。只有50-60%的化合物在2天内释放到介质中，这表明在几天内持续释放到伤口床。在大鼠实验全层创面模型上评价dbcamp掺入敷料的创面愈合效果。结果发现，敷料促进创面愈合，创面在10天内完全重新上皮化，而对照组创面则需要15天才能完全重新上皮化。本研究获得的数据表明，DBcAMP的存在加速了全层伤口的愈合和再上皮化。(c) 2005 Elsevier Ltd版权所有。
Cyclic adenosine monophosphate (cAMP) has long been regarded as a second messenger and a regulator of human keratinocyte proliferation. To explore more effective wound management, dibutyryl cyclic adenosine monophosphate (DBcAMP), a lipophilic analog of cAMP was incorporated into an in situ-forming hydrogel wound dressing based on periodate-oxidized alginate and gelatin. In vitro release of DBcAMP from the matrix into phosphate buffered saline was slow and increased with time. Only 50-60% of the compound was released into the medium over a period of 2 days suggestive of a sustained release into the wound bed over a period of few days. The wound-healing efficacy of the DBcAMP-incorporated dressing was evaluated on experimental full-thickness wounds in a rat model. It was found that dressing promoted wound healing leading to complete re-epithelialization of wounds within 10 days, whereas control wounds took 15 days for complete re-epithelialization. Data obtained in this Study showed that the presence of DBcAMP accelerated healing and re-epithelialization of full-thickness wounds. (c) 2005 Elsevier Ltd. All rights reserved.