Expression of Epiplakin1 in the developing and adult mouse retina
Expression of Epiplakin1 in the developing and adult mouse retina
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DOI：
10.1007/s10384-009-0751-y
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发表时间：
2010-02
影响因子：
2.4
通讯作者：
Tetsu Yoshida;Xiaoli Guo;K. Namekata;Y. Mitamura;S. Kume;T. Harada
中科院分区：
文献类型：
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作者：
Tetsu Yoshida;Xiaoli Guo;K. Namekata;Y. Mitamura;S. Kume;T. Harada
目的探讨Eppk1在发育中和成年小鼠视网膜中的表达。方法采用逆转录-聚合酶链式反应(RT-PCR)和免疫印迹法检测Eppk1的表达。用免疫组织化学方法检测胚胎和成年C57BL/6J小鼠视网膜中Eppk1的表达。Eppk1在胚胎视网膜中表达于巢蛋白阳性的神经前体细胞。在成人中，Eppk1在视网膜神经节细胞、双极细胞和Müler胶质细胞中均有表达。结论Eppk1在胚胎和成人视网膜中均有表达，可能参与视网膜的发育。
PurposeTo assess the expression of Epiplakin1 (Eppk1) in the developing and adult mouse retina.MethodsEppk1 expression was examined using reverse transcription-polymerase chain reaction (RTPCR) analysis and immunoblot analysis. The Eppk1 expression pattern was examined by immunohistochemical analysis in embryonic and adult C57BL/6J mice.ResultsBoth Eppk1 mRNA and protein were detected in adult mouse retina. Eppk1 was expressed in nestin-positive neural progenitor cells in the embryonic retina. In adults, Eppk1 was expressed in retinal ganglion cells, bipolar cells, and Müller glial cells.ConclusionsThese results suggest that Eppk1 is expressed both in embryonic and adult retina and may be involved in retinal development.