Histology and Mechanics of In Vivo Tissue-Engineered Vascular Graft for Children
Histology and Mechanics of In Vivo Tissue-Engineered Vascular Graft for Children
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DOI：
10.1016/j.athoracsur.2020.03.069
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发表时间：
2020-09-01
影响因子：
4.6
通讯作者：
Yaku, Hitoshi
中科院分区：
文献类型：
--
作者：
Fujita, Shuhei;Yamagishi, Masaaki;Yaku, Hitoshi
目的。本研究旨在评估用于儿童心脏手术的自体在体组织工程血管移植物的组织学和力学性能。2名男童于第一次手术时将硅胶引流管制成的活体TEVG模型植入皮下，第二次手术中作为补片材料用于治疗肺动脉狭窄。对其余的斑块进行组织学和机械学评价。在体内，TEVGs的管腔表面非常光滑，其管壁主要由胶原纤维和少量成纤维细胞组成。平均壁厚200 mm，平均缝线保持强度2.26N，破裂压力3057 mm Hg。人体内的TEVGs主要由胶原纤维组成，其力学性能证明其在肺动脉成形术中是安全的。因此，对于经常需要进行多阶段手术的儿童心血管外科手术，活体人体TEVGs可能是一种有前途的自体心包替代品。(C)2020年，由胸外科医生学会提供。
Purpose. This study sought to evaluate the histologic and mechanical properties of autologous in vivo tissue-engineered vascular grafts (in vivo TEVGs) used for pediatric heart surgery.Description. Molds of in vivo TEVGs made of silicone drain tubes were embedded into subcutaneous spaces in 2 boys during their first operation and were used as patch materials to treat pulmonary artery stenosis during the second operation. The remaining pieces of the patches were evaluated histologically and mechanically.Evaluation. In vivo TEVGs had very smooth luminal surfaces, and their walls mainly comprised collagen fibers and small numbers of fibroblasts. Mean wall thickness was 200 mm, mean suture retention strength was 2.26 N, and burst pressure was 3057 mm Hg.Conclusions. Human in vivo TEVGs mainly comprise collagen fibers, and their mechanical properties prove them safe for pulmonary arterioplasty. Therefore, human in vivo TEVGs may be promising alternatives to autologous pericardium for pediatric cardiovascular surgical procedures that often require multistage operations. (C) 2020 by The Society of Thoracic Surgeons.