Organoid Models for Cancer Research
Organoid Models for Cancer Research
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DOI：
10.1146/annurev-cancerbio-030518-055702
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发表时间：
2019-01-01
期刊：
影响因子：
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通讯作者：
Tuveson, David A.
中科院分区：
文献类型：
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作者：
Clevers, Hans;Tuveson, David A.
类器官培养已经成为加速细胞和癌症生物学发现的强大模型系统。这些三维培养物适用于多种技术，包括高通量基因组和转录组测序，以及遗传和生化扰动，使这些模型非常适合回答各种问题。最近，类器官已经从各种人类癌症中产生，包括乳腺癌、结肠癌、胰腺癌、前列腺癌、膀胱癌和肝癌，涉及这些模型的研究正在扩大我们对这些恶性肿瘤的病因和特征的了解。癌症类器官与非肿瘤基质细胞的共培养使得能够研究肿瘤微环境。此外，最近的研究已经确定，类器官在个性化医疗方法中占有一席之地。在这里，我们描述了类器官技术在癌症发现和治疗中的应用。
Organoid cultures have emerged as powerful model systems accelerating discoveries in cellular and cancer biology. These three-dimensional cultures are amenable to diverse techniques, including high-throughput genome and transcriptome sequencing, as well as genetic and biochemical perturbation, making these models well suited to answer a variety of questions. Recently, organoids have been generated from diverse human cancers, including breast, colon, pancreas, prostate, bladder, and liver cancers, and studies involving these models are expanding our knowledge of the etiology and characteristics of these malignancies. Co-cultures of cancer organoids with non-neoplastic stromal cells enable investigation of the tumor microenvironment. In addition, recent studies have established that organoids have a place in personalized medicine approaches. Here, we describe the application of organoid technology to cancer discovery and treatment.