Polyamine synthesis and accumulation in the castrated rat uterus after estradiol-17-beta stimulation.
Polyamine synthesis and accumulation in the castrated rat uterus after estradiol-17-beta stimulation.
复制标题
雌二醇-17-β刺激后去势大鼠子宫中多胺的合成和积累。
作者：
D. Russell;R. Taylor
在去势大鼠的子宫中研究了雌二醇-17β对多胺合成和积累的影响。单次注射 estradiol-17β 可使鸟氨酸脱羧酶 (ODC) 活性在 24 小时内增加 2 倍以上。检测到亚精胺合成酶 [腺苷-L-甲硫氨酸脱羧酶 (SAME)] 的显着升高，即 18 小时时，SAMD 活性比对照值高 8 倍。腐胺和亚精胺池的增加直接伴随着酶的暂时增加。 24 小时内腐胺浓度增加两倍，亚精胺浓度增加一倍。此后，腐胺和亚精胺浓度逐渐下降。该系统中的精胺水平也有所升高；它在24小时内达到比对照高2倍的水平，在72小时内达到峰值，此后显着下降，并且到96小时时没有显着高于对照。放线菌素 D 和放线菌酮基本上消除了 ODC 和 SAMD 活性的增加...
The effect of estradiol-17β on polyamine synthesis and accumulation was studied in the uterus of the castrated rat. A single injection of estradiol-17β produced more than a 2-fold increase in ornithine decarboxylase (ODC) activity within 24 hr. A more dramatic elevation of the spermidine synthesis enzyme [Sadenosyl- L-methionine decarboxylase (SAME)] was detected, i.e., SAMD activity was 8-fold higher than control values by 18 hr. Increased pools of putrescine and spermidine follow directly the temporal increases in the enzymes. Putrescine concentration tripled and spermidine concentration doubled within 24 hr. Thereafter, both putrescine and spermidine concentrations gradually decreased. The spermine level also was elevated in this system; it reached a level 2-fold higher than controls within 24 hr, peaked within 72 hr, and thereafter declined markedly and was not significantly higher than controls by 96 hr. Actinomycin D and cycloheximide essentially abolished the increases in both ODC and SAMD activiti...