Genetic analysis of the Salmonella enterica type III secretion-associated ATPase InvC defines discrete functional domains
Genetic analysis of the Salmonella enterica type III secretion-associated ATPase InvC defines discrete functional domains
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DOI：
10.1128/jb.186.8.2402-2412.2004
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发表时间：
2004-04-01
影响因子：
3.2
通讯作者：
Galán, JE
中科院分区：
文献类型：
--
作者：
Akeda, Y;Galán, JE
所有III型分泌系统的基本组分是高度保守的ATP酶，其与F0F1 ATP酶的β亚基具有显著的氨基酸序列相似性，并被认为为分泌过程提供能量。我们进行了InvC的遗传和功能分析，InvC是与肠道沙门氏菌III型分泌系统相关的ATP酶，编码在其致病岛1内。通过诱变分析，我们已经确定了氨基酸残基是必不可少的特定活动的InvC，如核苷酸水解和膜结合。这使我们能够定义InvC的离散结构域，这些结构域与这种蛋白质的不同基本活性特异性相关。
An essential component of all type III secretion systems is a highly conserved ATPase that shares significant amino acid sequence similarity to the beta subunit of the F0F1 ATPases and is thought to provide the energy for the secretion process. We have performed a genetic and functional analysis of InvC, the ATPase associated with the Salmonella enterica type III secretion system encoded within its pathogenicity island 1. Through a mutagenesis analysis, we have identified amino acid residues that are essential for specific activities of InvC, such as nucleotide hydrolysis and membrane binding. This has allowed us to define discrete domains of InvC that are specifically associated with different essential activities of this protein.