Phytosterols from Dunaliella tertiolecta and Dunaliella sauna: A potentially novel industrial application
Phytosterols from Dunaliella tertiolecta and Dunaliella sauna: A potentially novel industrial application
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DOI：
10.1016/j.biortech.2009.12.139
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发表时间：
2010-06-01
影响因子：
11.4
通讯作者：
Luque, Rafael
中科院分区：
文献类型：
--
作者：
Francavilla, Matteo;Trotta, Pasquale;Luque, Rafael
从三聚杜氏藻和盐杜氏藻中提取并分析了甾醇，以评价这些微藻作为植物甾醇来源的潜在新型工业开发。研究了盐浓度对甾醇产率的影响。通过气相色谱-质谱联用分析，鉴定出12种甾醇。植物甾醇含量最高的是(22E,24R)-甲基胆固醇-5,7,22-三烯-3 - β -醇（麦角甾醇）和(22E,24R)-乙基胆固醇-5,7,22-三烯-3 - β -醇（7-脱氢多孔甾醇）。整个甾醇部分主要由植物甾醇（C（28）和C（29）甾醇）组成。较低盐浓度下总甾醇产量较好（0.6 M NaCl下，三叶菜和盐菜分别占干重的1.3%和0.89%），而盐浓度的增加导致总甾醇产量显著降低。(C) 2010 Elsevier Ltd.版权所有。
Sterols have been extracted and analysed from Dunaliella tertiolecta and Dunaliella salina, in order to evaluate a potentially novel industrial exploitation of these microalgae as source of phytosterols. The effect of salt concentration on sterols yields has been studied varying the quantities of NaCl into culture medium. Twelve sterols were identified by Gas-chromatographic MS/MS analysis for both algal strains. The most abundant phyrosterols were (22E,24R)-methylcholesta-5,7,22-trien-3 beta-ol (ergosterol) and (22E,24R)-ethylcholesta-5,7,22-trien-3 beta-ol (7-dehydroporiferasterol). The whole sterol fraction consisted mainly of phytosterols (C(28) and C(29) sterols). Good yields of total sterols were achieved at lower salt concentration (1.3% and 0.89% of dry weight in D. tertiolecta and D. salina, respectively, at 0.6 M NaCl), while an increase in salt concentration resulted in a significant decrease in total sterols yield. (C) 2010 Elsevier Ltd. All rights reserved.