The changes of GA level and signaling are involved in the regulation of mesocotyl elongation during blue light mediated de-etiolation in Sorghum bicolor
The changes of GA level and signaling are involved in the regulation of mesocotyl elongation during blue light mediated de-etiolation in Sorghum bicolor
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蓝光介导的高粱去黄化过程中GA水平和信号传导的变化参与中胚轴伸长的调节
DOI：
10.1007/s11033-011-1191-6
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发表时间：
2011-07
期刊：
影响因子：
--
通讯作者：
王小菁
中科院分区：
文献类型：
--
作者：
王小菁
幼苗发育过程中的脱黄化受蓝光(BL)和赤霉素(GAs)拮抗调节。蓝光(BL)与GA代谢和信号之间的串扰仍不清楚。利用对BL敏感的突变体HAR1进行脱黄化处理，探讨GA代谢、GA信号转导在抑制高粱中胚轴伸长中的作用。R111)进行了地膜下播种试验。外源GA(3)可恢复BL对HAR1中胚轴和细胞伸长的抑制作用。BL光照射后1~4h，HAR1中胚轴内源GA(3)水平相应降低。用实时荧光定量聚合酶链式反应检测了赤霉素A代谢酶SbGA20ox、SbGA3ox和SbGA2ox的可能基因，结果表明其中一个SbGA2ox同源物在BL2 h时在HAR1中的转录水平显著高于在R111中的转录水平。BL光照射后，DELLAS的同源基因增加，其中HAR1的同源基因较高。外源多效唑(PAC)对Della的表达有明显的促进作用。我们的研究为单子叶高粱提供了证据，表明一组GA代谢和信号基因的变化可能参与了BL诱导的细胞伸长抑制。
De-etiolation during seedling development is antagonistically regulated by blue light (BL) and gibberellins (GAs). The crosstalk between blue light (BL) and GA metabolism and signaling remains unclear. Using the mutant har1 which is specifically hypersensitive to BL in de-etiolation, the involvement possibility of the GA metabolism, GA signaling in the inhibition of mesocotyl elongation of the sorghum (Sorghum bicolor L. var. R111) seeding under BL was investigated. The inhibition of mesocotyl and cell elongation by BL was restored by application of exogenous GA(3) in har1. The endogenous GA(3) level correspondingly decreased in har1 mesocotyl especially from 1 to 4 h after BL irradiation. Putative genes of GA metabolism enzymes SbGA20ox, SbGA3ox and SbGA2ox were detected by Real-Time PCR and the results showed that one of the SbGA2ox homologs appeared significantly higher transcript level in har1 than in R111 at 2 h after BL irradiation. Putative homologous genes of DELLAs increased after BL irradiation and were higher in har1 among the three homologs. Remarkable increase of the DELLA expression was observed responding to exogenous paclobutrazol (PAC). Our research provided evidence in monocot sorghum, that the changes of a set of the GA metabolism and signaling genes might be involved in BL-induced inhibition of cell elongation.
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影响因子：
4.3
作者：
G. M. Symons;J. B. Reid
通讯作者：
G. M. Symons;J. B. Reid
影响因子：
9.5
作者：
C. Schwechheimer;B. Willige
通讯作者：
C. Schwechheimer;B. Willige
影响因子：
7.4
作者：
M. Lozano-Baena;E. Prats;M. Moreno;D. Rubiales;A. Pérez-de-Luque
通讯作者：
M. Lozano-Baena;E. Prats;M. Moreno;D. Rubiales;A. Pérez-de-Luque
DOI：
10.1073/pnas.95.5.2686
发表时间：
1998-03-03
影响因子：
11.1
作者：
Lin, CT;Yang, HY;Cashmore, AR
通讯作者：
Cashmore, AR
影响因子：
56.9
作者：
Liu, Hongtao;Yu, Xuhong;Lin, Chentao
通讯作者：
Lin, Chentao