Identification and phylogeny of Arabian snakes: Comparison of venom chromatographic profiles versus 16S rRNA gene sequences
Identification and phylogeny of Arabian snakes: Comparison of venom chromatographic profiles versus 16S rRNA gene sequences
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DOI：
10.1016/j.sjbs.2014.05.002
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发表时间：
2014-11-01
影响因子：
4.4
通讯作者：
Khan, Haseeb Ahmad
中科院分区：
文献类型：
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作者：
Al Asmari, Abdulrahman;Manthiri, Rajamohammed Abbas;Khan, Haseeb Ahmad
由于各种原因，包括对蛇咬伤受害者的紧急治疗，蛇的种类识别很重要。提出了一种利用蛇毒凝胶过滤色谱图鉴别六种蛇类的简便方法。将Echis coloratus、Echis spermidum、Cerastes gasperettii、Bitis arietans、Naja arabica和Walterinnesia aegyptia的毒液挤奶、冻干、稀释和离心以将粘液与毒液分离。将澄清的上清液过滤并在快速蛋白质液相色谱（FPLC）上进行色谱分离。我们获得了上述物种的16 S rRNA基因序列，并进行了系统发育分析，使用相邻连接的方法。不同蛇种毒液的色谱图显示出基于峰的数量和位置的特殊模式。基于存在/不存在特定色谱峰的相似性矩阵生成的树状图清楚地区分了Elapids和Viperids。使用16 S rRNA基因序列的分子分支导致跳跃的分支，而分离这两个家庭的成员。这些结果表明，蛇毒的色谱图可以提供一个简单的和重复性的化学指纹图谱的快速鉴定蛇类的方法。然而，该方法的验证需要对来自物种内和物种间的大量标本进行进一步研究。(C)2014年由Elsevier B. V.代表沙特国王大学制作和主持。
Identification of snake species is important for various reasons including the emergency treatment of snake bite victims. We present a simple method for identification of six snake species using the gel filtration chromatographic profiles of their venoms. The venoms of Echis coloratus, Echis pyramidum, Cerastes gasperettii, Bitis arietans, Naja arabica, and Walterinnesia aegyptia were milked, lyophilized, diluted and centrifuged to separate the mucus from the venom. The clear super-natants were filtered and chromatographed on fast protein liquid chromatography (FPLC). We obtained the 16S rRNA gene sequences of the above species and performed phylogenetic analysis using the neighbor-joining method. The chromatograms of venoms from different snake species showed peculiar patterns based on the number and location of peaks. The dendrograms generated from similarity matrix based on the presence/absence of particular chromatographic peaks clearly differentiated Elapids from Viperids. Molecular cladistics using 16S rRNA gene sequences resulted in jumping clades while separating the members of these two families. These findings suggest that chromatographic profiles of snake venoms may provide a simple and reproducible chemical fingerprinting method for quick identification of snake species. However, the validation of this methodology requires further studies on large number of specimens from within and across species. (C) 2014 Production and hosting by Elsevier B.V. on behalf of King Saud University.