Targeted gene knockout of inner arm 1 in Tetrahymena thermophila
Targeted gene knockout of inner arm 1 in Tetrahymena thermophila
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DOI：
10.1078/0171-9335-00178
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发表时间：
2001-07-01
影响因子：
6.6
通讯作者：
Pennock, DG
中科院分区：
文献类型：
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作者：
Angus, SP;Edelmann, RE;Pennock, DG
纤毛和鞭毛包含至少八种不同类型的动力臂。目前还不完全清楚不同类型的臂是如何沿着轴突组织的。此外，每种不同类型的动力蛋白在纤毛或鞭毛运动中所起的作用尚不清楚。为了开始研究动力蛋白在纤毛中的组织和功能，我们通过靶向基因敲除在嗜热四膜虫中引入一个动力蛋白重链基因（DYH6）突变。我们已经产生了缺乏DYH6基因野生型拷贝的突变细胞。我们已经证明DYH6基因编码四膜虫18S动力蛋白的一条重链（HC2），并且18S动力蛋白位于纤毛轴突的I1位置。我们还表明，正常运动、正常摄食和正常加倍率都需要四膜虫I1。
Cilia and flagella contain at least eight different types of dynein arms. It is not entirely clear how the different types of arms are organized along the axoneme. In addition, the role each different type of dynein plays in ciliary or flagellar motility is not known. To initiate studies of dynein organization and function in cilia, we have introduced a mutation into one dynein heavy chain gene (DYH6) in Tetrahymena thermophila by targeted gene knockout. We have generated mutant cells that lack wild-type copies of the DYH6 gene. We have shown that the DYH6 gene encodes one heavy chain (HC2) of Tetrahymena 18S dynein and that 18S dynein occupies the I1 position in the ciliary axoneme. We have also shown that Tetrahymena I1 is required for normal motility, normal feeding and normal doubling rate.