"Turn-off" photoelectrochemical aptasensor based on g-C3N4/WC/WO3 composites for tobramycin detection
"Turn-off" photoelectrochemical aptasensor based on g-C3N4/WC/WO3 composites for tobramycin detection
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DOI：
10.1016/j.foodchem.2022.134287
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发表时间：
2022-09-29
期刊：
影响因子：
8.8
通讯作者：
Zhang, Chang
中科院分区：
文献类型：
--
作者：
Qiao, Lu;Zhu, Yuan;Zhang, Chang
妥布霉素（Tobramycin，TOB）作为一种广泛使用的抗生素，对生态和健康构成严重的不可预测的风险。在这里，一种新的光电化学（PEC）适体传感器的基础上的“关闭”PEC模式被构建用于TOB检测。工作电极可以依次用g-C3 N4/WC/WO 3复合材料和TOB适配子探针修饰。TOB适体探针可以通过7 C-7 C堆积相互作用锚在g-C3 N4/WC/WO 3上，以避免其他修饰的干扰。当TOB被锚定在修饰的掺氟氧化锡（FTO）电极上的适体探针捕获时，由于空间位阻和阻碍电子转移，获得了降低的光电流。在最佳条件下，0.005-5 ng mL-1的TOB可以检测到低至2 pg mL-1的检测限。同时，对实际样品进行了研究。该传感器具有特异性强、检测灵敏度高、重现性好等特点，为其他污染物的检测提供了一种新的思路。
Tobramycin (TOB), as a widely used antibiotic, poses severe unpredictable risks to ecology and health. Here, a novel photoelectrochemical (PEC) aptasensor based on a "turn-off" PEC mode was constructed for TOB detection. The working electrode could be modified by g-C3N4/WC/WO3 composites and TOB aptamer probes in turn. The TOB aptamer probes could anchor on the g-C3N4/WC/WO3 through 7C-7C stacking interaction to avoid interference from other modifications. When TOB was captured by the aptamer probes anchored on the modified fluorine -doped tin oxide (FTO) electrode, a decreased photocurrent was obtained owing to steric hindrance and hinder electron transfer. Under optimal conditions, 0.005-5 ng mL-1 of TOB could be detected with a detection limit as low as 2 pg mL-1. Meanwhile, actual samples were investigated as well. The proposed sensor shows high specificity, satisfactory detectability, great reproducibility, and may provide a new thought for detecting other pollutants.