Manganese oxidation state as a cause of irritant patch test reactions.
Manganese oxidation state as a cause of irritant patch test reactions.
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DOI：
10.1097/der.0000000000000032
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发表时间：
2014-03
期刊：
影响因子：
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通讯作者：
Gaspari AA
中科院分区：
文献类型：
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作者：
Shallcross L;Ritchie S;Harberts E;Tammaro A;Gaitens J;Gaspari AA
2.5%氯化锰（MnCl2）被纳入扩展金属斑贴试验系列，以评价患者对该金属盐的接触性超敏反应。本研究的目的是前瞻性确定接受斑贴试验的患者队列中MnCl2（Mn（II））、Mn2O3（Mn（III））和KMnO 4（Mn（VII））的过敏性和刺激性斑贴试验反应的发生率。58名患者接受了MnCl2、Mn2O3和KMnO4的斑贴试验，每种都在凡士林中占2.5%。在48小时、72小时或96小时采集贴片读数，并使用标准方法进行评分。角质形成细胞（KCs）培养单层暴露于MnCl2，Mn2O3，和KMnO4在水性培养基中，细胞存活和细胞因子释放进行了研究。MnCl2在41%的队列中引起刺激性斑贴试验反应，而Mn2O3和KMnO4引起的刺激性反应率显著较低（均为3%）。未观察到过敏形态。同样，在培养的KC单层中，只有MnCl2对KC具有细胞毒性并诱导肿瘤坏死因子α释放。用于斑贴试验的锰的氧化态会影响该金属盐的刺激性，因为Mn（II）在一组患者中引起了不可接受的高刺激性反应率。体外研究证实了这些临床数据，因为只有Mn（II）对培养的KC单层细胞具有细胞毒性。
Manganese chloride (MnCl2) 2.5% is included in the extended metals patch test series to evaluate patients for contact hypersensitivity to this metal salt. The objective of this study was to prospectively determine the rate of allergic and irritant patch test reactions to MnCl2 (Mn(II)), Mn2O3 (Mn(III)), and KMnO4 (Mn(VII)) in a cohort of patients undergoing patch testing. Fifty-eight patients were patch tested with MnCl2, Mn2O3, and KMnO4, each at 2.5% in petrolatum. Patch readings were taken at 48, and 72 or 96 hours, and scored using standard methods. Cultured monolayers of keratinocytes (KCs) were exposed to MnCl2, Mn2O3, and KMnO4 in aqueous culture medium, and cell survival and cytokine release were studied. MnCl2 caused irritant patch test reactions in 41% of the cohort, whereas Mn2O3 and KMnO4 caused a significantly lower rate of irritant reactions (both 3%). No allergic morphologies were observed. Similarly, in cultured KC monolayers, only MnCl2 was cytotoxic to KC and induced tumor necrosis factor α release. The oxidation state of manganese used for patch testing affects the irritancy of this metal salt, as Mn(II) caused an unacceptably high rate of irritant reactions in a cohort of patients. In vitro studies confirmed these clinical data, as only Mn(II) was cytotoxic to cultured monolayers of KC.