Genome-Wide Profiling of Human Papillomavirus DNA Integration into Human Genome and Its Influence on PD-L1 Expression in Chinese Uygur Cervical Cancer Women
Genome-Wide Profiling of Human Papillomavirus DNA Integration into Human Genome and Its Influence on PD-L1 Expression in Chinese Uygur Cervical Cancer Women
复制标题
DOI：
10.1155/2020/6284960
复制
发表时间：
2020-01-23
影响因子：
4.1
通讯作者：
Huang Yan-chun
中科院分区：
文献类型：
--
作者：
Feng Yang-chun;Wang Sen-yu;Huang Yan-chun
背景。维吾尔族是中国人口第五大民族。与中国其他人群相比，宫颈癌发病率较高，HPV感染也较为特殊。他们的HPV整合情况从未报道过。目的探讨中国维吾尔族宫颈癌患者中20个亚型HPV基因在宿主细胞基因组中的整合情况；同时，我们探讨了基因整合对PD-L1表达的影响。方法：从新疆医科大学附属肿瘤医院肿瘤防治所采集HPV感染阳性的中国维吾尔族宫颈癌冷冻标本40例。采用Agilent SureSelect （TM）靶向富集芯片和下一代测序技术检测HPV基因与宿主细胞基因组的整合情况。通过GO功能注释和KEGG通路富集分析相关基因。采用免疫组化法检测PD-L1在肿瘤细胞中的表达水平。同时分析了癌细胞中PD-L1水平与基因整合的关系。结果。HIVID的HPV多重感染率高达92.5%，远高于市售试剂盒的35.0% (P
Background. The Uygur is the fifth most populous ethnic group in China. Compared to other Chinese population, cervical cancer in them had high incidence, and HPV infection also was particular. Their HPV integration situation has never been reported. We aimed to investigate the integration situation of 20 subtypes of HPV gene into host cell genome in Chinese Uygur cervical cancer patients; meanwhile, we explored the influence of gene integration on PD-L1 expression. Methods. 40 frozen Chinese Uygur cervical cancer specimens with positive HPV infection were obtained from the cancer prevention and treatment institute of Tumor Hospital Affiliated to Xinjiang Medical University. The integration situation of HPV gene into host cell genome was detected by Agilent SureSelect (TM) Target Enrichment Chip and Next-Generation Sequencing. The related genes were analyzed by GO functional annotation and KEGG pathway enrichment. The expression levels of PD-L1 in cancer cells were tested by immunohistochemical assay (IHC). Meanwhile, the relationship between PD-L1 levels in cancer cells and gene integration were analyzed. Results. The HPV multiple infection rate by HIVID was as high as 92.5%, much higher than 35.0% by the commercial kit (P