Identification and sequence analysis of alkaloid biosynthesis genes in Papaver section Oxytona
Identification and sequence analysis of alkaloid biosynthesis genes in Papaver section Oxytona
复制标题
DOI：
10.3906/biy-1505-22
复制
发表时间：
2016-01-01
影响因子：
2.2
通讯作者：
Unver, Turgay
中科院分区：
文献类型：
--
作者：
Alagoz, Yagiz;Gurkok, Tugba;Unver, Turgay
苄基异喹啉生物碱（BIA）是由包括大苞罂粟（Papaver bracteatum）、伪东方罂粟（Papaver pseudo-orientale）和东方罂粟（Papaver orientale）（Oxytona Bernh组的三个代表性成员）在内的许多罂粟属物种产生的次级代谢产物。定位在BIA生物合成途径上的基因的序列和表达水平先前在罂粟（罂粟）中被定义，罂粟是同属中研究最多的物种之一。然而，大多数预测的BIA相关的基因序列在部分Oxytona尚未指定。在这里，我们提出了新的cDNA序列，属于Oxytona物种。本研究对拟东方扁藻的去甲乌药碱6-O-甲基转移酶（6 OMT）、NADPH依赖的可待因酮还原酶（COR）、salutaridinol 7-O-乙酰转移酶（SalAT）和（S）-四氢原小檗碱顺式-N-甲基转移酶（TNMT）、大苞扁藻的3-羟基-N-甲基乌药碱4 '-O-甲基转移酶（4 OMT）、首次在东方扁藻中鉴定出TNMT和6 OMT基因，并对部分已测序的基因进行了重新测序。此外，还通过qRT-PCR检测了这些基因在叶片中的表达。这些发现被用来构建系统发育树，展示BIA相关基因的进化关系之间的Oxytona物种。
The benzylisoquinoline alkaloids (BIA) are secondary metabolites that are produced by many of the Papaver species including Papaver bracteatum, Papaver pseudo-orientale, and Papaver orientale, three representative members of the section Oxytona Bernh. The sequences and expression levels of the genes positioned on the BIA biosynthesis pathway were previously defined in opium poppy (Papaver somniferum), one of the mostly studied species of the same genus. Nevertheless, the majority of predicted BIA-related gene sequences in the section Oxytona have not been specified. Here we are presenting new cDNA sequences that belong to the Oxytona species. In this study, partial sequences of norcoclaurine-6-O-methyltransferase (6OMT), NADPH-dependent codeinone reductase (COR), salutaridinol 7-O-acetyltransferase (SalAT), and (S)-tetrahydroprotoberberine cis-N-methyltransferase (TNMT) in P. pseudo-orientale; 3-hydroxy-N-methylcoclaurine 4'-O-methyltransferase (4OMT) in P. bracteatum; and TNMT and 6OMT in P. orientale were identified for the first time, and some of the previously sequenced genes were resequenced in the section Oxytona. Furthermore, expressions of those genes were also detected by using qRT-PCR in leaves. The findings were used to construct phylogenetic trees demonstrating the evolutionary relationships of BIA-related genes among Oxytona species.