Both nerve growth factor and high K+ concentrations support the survival of chick embryo sympathetic neurons. Evidence for a common mechanism of action.
Both nerve growth factor and high K+ concentrations support the survival of chick embryo sympathetic neurons. Evidence for a common mechanism of action.
复制标题
神经生长因子和高浓度 K 都支持鸡胚交感神经元的存活。
DOI：
10.1016/0014-4827(83)90417-2
复制
发表时间：
1983
影响因子：
3.7
通讯作者：
Thoenen,H
中科院分区：
文献类型：
--
作者：
Wakade,AR;Edgar,D;Thoenen,H
从鸡胚的交感神经节中分离神经元，并在不存在非神经元细胞的情况下进行培养。神经生长因子（NGF）和高浓度的细胞外K+支持神经元的存活，这些影响是独立的血清在培养基中的存在。只有60%的神经元存活在35 mM的K+，和生存没有增加时，神经生长因子和K+一起存在。然而，如果培养基质用心脏细胞条件培养基（其本身不支持神经元存活）预处理，则有可能在具有NGF或高K+浓度的培养物中维持基本上所有神经元。这些观察结果与K+和NGF对培养的胚胎神经元存活的共同作用机制一致。
Neurons were dissociated from the sympathetic ganglia of embryonic chicks, and cultured in the absence of non-neuronal cells. Both nerve growth factor (NGF) and high concentrations of extracellular K+supported neuronal survival, and these effects were independent of the presence of serum in the culture medium. Only 60% of the neurons survived in response to 35 mM K+, and survival was not increased when both NGF and K+were present together. It was, however, possible to maintain essentially all the neurons in culture with either NGF or high K+concentrations if the culture substrate had been pretreated with heart cell-conditioned medium (which did not itself support neuronal survival). These observations are consistent with a common mechanism of action of both K+and NGF for the survival of cultured embryonic neurons.