Distribution of Protists in the Deep South China Sea Revealed by High-Throughput Sequencing
Distribution of Protists in the Deep South China Sea Revealed by High-Throughput Sequencing
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DOI：
10.1007/s11802-020-4137-6
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发表时间：
2019-11
影响因子：
1.6
通讯作者：
Xinran Li;A. Warren;N. Jiao;Dapeng Xu
中科院分区：
文献类型：
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作者：
Xinran Li;A. Warren;N. Jiao;Dapeng Xu
原生生物（微生物真核生物）是海洋微生物食物网中不可或缺的成员。近年来，生活在深海（>1000 m水深）的生物越来越成为研究的焦点，但与原核生物相比，对原生生物组合的研究相对较少。本研究利用18 S rRNA基因V9区高变区的高通量测序技术，对南海深层沃茨原生生物群落组成进行了研究。通过对14个样品的α和β多样性分析，发现：1）Rhizaria、Alveolata和Excavata的相对序列丰度和OTU丰度在所有样品中均占优势，但不同样品中它们的相对贡献有所不同; 2）聚类分析表明，原生动物群落的分布与取样地点和水深无关，可能是其他环境因素造成的;（3）在所有样品中均检测到光养生物，包括硅藻门、藻藻门、网枝藻门、Prasinophyceae和Prymnesiophyceae，表明它们参与了深海中的光合生物泵，并可能参与了这些光养细胞的摄食。
Protists (microbial eukaryotes) are indispensable members of the marine microbial food web. In recent years, organisms living in the deep sea (>1000 m water depth) have increasingly become the focus of research; however, studies on protistan assemblages are relatively scarce compared with their prokaryotic counterparts. In the present study, high-throughput sequencing of the hypervariable V9 region of the 18S rRNA gene was used to explore the community composition of protists in bathypelagic waters of the South China Sea. Based on the analysis of the alpha and beta diversities of 14 samples, we discovered: 1) members belonging to Rhizaria, Alveolata, and Excavata were the dominant groups in terms of both relative sequence abundance and operational taxonomic unit (OTU) richness in all samples, although their relative contributions differed among different samples; 2) cluster analysis showed that the distribution of protistan assemblages was related neither to the sampling location nor to the water depth, and other environmental factors might have caused the differences among the communities; 3) phototrophs, including members of the Bacillariophyta, Bolidophyceae, Dictyochophyceae, Prasinophyceae, and Prymnesiophyceae, were detected in all samples, which indicated their contributions to the downward transportationviathe biological pump and the potential presence of phagotrophy of these phototrophic cells in the deep ocean.