A comparison between the phenol-chloroform method of RNA extraction and the QIAamp viral RNA kit in the extraction of hepatitis C and GB virus-C/hepatitis G viral RNA from serum
A comparison between the phenol-chloroform method of RNA extraction and the QIAamp viral RNA kit in the extraction of hepatitis C and GB virus-C/hepatitis G viral RNA from serum
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DOI：
10.1016/s0166-0934(00)00192-0
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发表时间：
2000-09-01
影响因子：
3.1
通讯作者：
Di Bisceglie, AM
中科院分区：
文献类型：
--
作者：
Fanson, BG;Osmack, P;Di Bisceglie, AM
用于临床和研究目的时，通常通过杂交和聚合酶链式反应 (PCR) 方法检测血清中的病毒 RNA。在 PCR 扩增之前评估了两种提取病毒 RNA 的方法。一种是传统的苯酚-氯仿方法，另一种使用标准化的制造套件。通过丙型肝炎病毒和 GB 病毒-丙型/庚型肝炎病毒 RNA 的半定量扩增来测试提取效率。标准化的商业方法虽然更省时，但导致灵敏度降低了大约十倍。因此，在需要最大灵敏度的情况下，建议使用劳动强度更大的苯酚氯仿法。 (C) 2000 Elsevier Science B.V. 保留所有权利。
Viral RNAs are detected commonly in serum by hybridization and polymerase chain reaction (PCR) methods for both clinical and research purposes. Two methods of extracting Viral RNA were evaluated prior to amplification by PCR. One was a conventional phenol-chloroform method and the other used a standardized, manufactured kit. The efficiency of extraction was tested by semi-quantitative amplification of hepatitis C viral and GB virus-C/hepatitis G viral RNAs. The standarized commercial method, although more time efficient, resulted in an approximately ten fold less sensitivity. Thus, in situations where maximum sensitivity is needed, the more labor intenstive phenol choloroform method is recommended. (C) 2000 Elsevier Science B.V. All rights reserved.