MITOTIC ACTIVITY AND SPECIFICATION OF FIBROCYTE SUBTYPES IN THE DEVELOPING RAT COCHLEAR LATERAL WALL
MITOTIC ACTIVITY AND SPECIFICATION OF FIBROCYTE SUBTYPES IN THE DEVELOPING RAT COCHLEAR LATERAL WALL
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DOI：
10.1016/j.neuroscience.2009.07.059
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发表时间：
2009-11-10
期刊：
影响因子：
3.3
通讯作者：
Matsunaga, T.
中科院分区：
文献类型：
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作者：
Mutai, H.;Nagashima, R.;Matsunaga, T.
乳房中的螺旋韧带纤维细胞（SLF）。耳蜗外侧壁参与K+循环，根据形态、分布和功能可分为5个亚型。SLF的再生是纠正几种类型听力损失的潜在治疗策略，这促使我们研究SLF亚型在发育过程中是如何建立的。我们比较了过渡SLF型标志物表达与有丝分裂活性，以评估出生后大鼠耳蜗SLF的增殖-分化关系。腹腔注射5-溴-2 '-脱氧尿苷（BrdU）表明，出生后第7天（P7）和P10之间SLF的总体有丝分裂活性显著降低。对于所有的发展时期，BrdU掺入是最弱的I型SLF居住的地区。II/IV型SLF标志物的表达开始于细胞有丝分裂活性降低之后，而III型SLF标志物水通道蛋白-1的表达在P7时已经可检测到，此时III型SLF仍在剧烈增殖。在P7和P10之间，BrdU（+）细胞在I型SLF区域的分布增加，表明SLF从邻近区域迁移。我们的结论是SLF的发展的时间过程是亚型特异性的。(C)2009年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Spiral ligament fibrocytes (SLFs) in the mamma. lian cochlear lateral wall participate in K+ recycling; they are classified into five subtypes based on their morphology, distribution, and function. Regeneration of SLFs is a potential therapeutic strategy for correcting several types of hearing loss, prompting us to investigate how SLF subtypes are established during development. We compared transitional SLF-type marker expression with mitotic activity to evaluate proliferation-differentiation relationships in SLFs from postnatal rat cochleae. I.p. injection of 5-bromo-2'-deoxyuridine (BrdU) demonstrated that the overall mitotic activity of SLFs decreased significantly between postnatal day 7 (P7) and P10. For all developmental periods, BrdU incorporation was weakest in the area where type I SLFs reside. The onset of expression of markers for type II/IV SLFs followed the reduced mitotic activity of the cells, whereas that of aquaporin-1, a marker for type III SLFs, was already detectable at P7, when the type III SLFs were still proliferating vigorously. Distribution of BrdU(+) cells increased in the area of type I SLFs between P7 and P10, suggesting migration of SLFs from adjacent areas. We conclude that the time course of development of SLFs is subtype-specific. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.