ISOZYME VARIABILITY AND DIFFERENTIATION BETWEEN RHODNIUS-PROLIXUS, R-ROBUSTUS AND R-PICTIPES, VECTORS OF CHAGAS-DISEASE IN VENEZUELA
ISOZYME VARIABILITY AND DIFFERENTIATION BETWEEN RHODNIUS-PROLIXUS, R-ROBUSTUS AND R-PICTIPES, VECTORS OF CHAGAS-DISEASE IN VENEZUELA
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DOI：
10.1111/j.1365-2915.1992.tb00032.x
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发表时间：
1992-01-01
影响因子：
1.9
通讯作者：
CARIOU, ML
中科院分区：
文献类型：
--
作者：
HARRY, M;GALINDEZ, I;CARIOU, ML
用淀粉和聚丙烯酰胺凝胶电泳分析了南美锥蝽属（半翅目：猎蝽科）锥蝽的酶多态性。 结果表明，在所检测的45种酶中，有17种酶（AO、CA、DIA、ES、ES-A、FH、GPD、G6 PD、GPI、MDH、ME、6PGD、PGM、ACON、ACPH、SOD）的电泳图谱可评分，涉及22个结构位点。 因此，这些基因-酶系统被选择用于R.不同品系的prolixus Stal成虫。 对三种虫的前13种酶，包括16个结构位点进行了分析。prolixus、河robustus Larrousse和R.皮克提普斯湾 计算了三个R. prolixus菌株：3 - 5个位点表现出多态性。 多态位点比例（22%）和平均杂合度（0.06）表明，遗传变异较低，在个别位点的菌株之间的差异显着。 Rhodnius prolixus和R.结果表明，两种植物具有相同的同工酶谱。 R. pictipes遗传分化良好，有12个诊断位点。
Enzyme polymorphism in triatomine bugs of the genus Rhodnius (Hemiptera: Reduviidae), vectors of Chagas disease, is analysed using both starch and polyacrylamide gel electrophoresis. Out of forty-five enzymes assayed, the electromorphs of seventeen of them: AO, CA, DIA, ES, ES-A, FH, GPD, G6PD, GPI, MDH, ME, 6PGD, PGM, ACON, ACPH, LAP and SOD, involving twenty-two putative structural loci, were scorable. These gene-enzyme systems were therefore selected for routine characterization of R. prolixus Stal adults from different strains. The first thirteen enzymes, involving sixteen structural loci, were also analysed in first instar nymphs of the three species, R. prolixus, R. robustus Larrousse and R. pictipes Stal. Allelic frequencies were calculated for three R. prolixus strains: three to five loci appeared to be polymorphic. The proportion of polymorphic loci (22%) and the average heterozygosity (0.06) indicated low genetic variability, with significant differences between the strains at individual loci. Rhodnius prolixus and R. robustus were found to have identical isozymic patterns. R. pictipes was genetically well differentiated, with twelve diagnostic loci.