Canine invasive transitional cell carcinoma cell lines: In vitro tools to complement a relevant animal model of invasive urinary bladder cancer
Canine invasive transitional cell carcinoma cell lines: In vitro tools to complement a relevant animal model of invasive urinary bladder cancer
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DOI：
10.1016/j.urolonc.2008.02.015
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发表时间：
2009-05-01
影响因子：
2.7
通讯作者：
Knapp, Deborah W.
中科院分区：
文献类型：
--
作者：
Dhawan, Deepika;Ramos-Vara, Jose A.;Knapp, Deborah W.
目的：膀胱癌是美国人类最常见的第五种癌症。膀胱癌也发生在宠物狗身上，而宠物狗的自然发生的膀胱癌与人类的浸润性膀胱癌(中高度侵袭性移行细胞癌，InvTCC)非常相似。带有InvTCC的宠物狗为膀胱癌的临床前研究提供了高度相关的资源。对于将研究结果从体外实验、狗体内研究转移到人体试验的转化型研究来说，获得人类和狗的膀胱癌细胞株是重要的。犬InvTCC来源的细胞系一直缺乏。本文描述了8个这样的细胞系。材料和方法：从犬的InvTCC中建立了8个细胞系。用免疫细胞化学方法对细胞进行鉴定，评估细胞在软琼脂中的贴壁非依赖性生长，并评估裸鼠的致瘤性。Western blotting鉴定目的蛋白在人InvTCC中的表达。结果：通过细胞角蛋白和E-钙粘蛋白的表达，证实细胞系为上皮源性。在裸鼠体内发现有7个细胞系具有致瘤性，其中4个细胞系以非锚定方式生长。结论：所建立的细胞系可用于膀胱癌的体外比较研究，并在体内试验前评估新的治疗方法。(C)2009 Elsevier Inc.保留所有权利。
Objectives: Urinary bladder cancer is the fifth most common form of cancer in humans in the United States. Urinary bladder cancer also occurs in pet dogs, and naturally-occurring bladder cancer in pet dogs very closely resembles invasive bladder cancer (intermediate to high grade invasive transitional cell carcinoma, InvTCC) in humans. Pet dogs with InvTCC offer a highly relevant resource for preclinical Studies in bladder cancer. For translational research in which findings are moved from in vitro experiments through in vivo studies in dogs to human trials, access to human and canine bladder cancer cell lines is important. Cell lines derived from canine InvTCC have been lacking. Here we describe eight such cell lines.Materials and methods: Eight cell lines were established from canine InvTCC. Cells were characterized using immunocytochemistry, evaluated for anchorage independent growth in soft agar, and assessed for tumorigenicity in athymic mice. Western blotting was used to identify expression of proteins of interest in human InvTCC.Results: The cell lines were confirmed to be of epithelial origin by their expression of cytokeratin and E-cadherin. Seven cell lines were found to be tumorigenic in athymic mice, and 4 of these cell lines grew in an anchorage independent manner. The cell lines expressed several proteins of interest associated with bladder cancer prognosis and progression in humans, including p53, cox-2, and pRb protein.Conclusions: These established cell lines can be used for comparative bladder cancer research and to evaluate new therapy approaches in vitro prior to in vivo testing. (C) 2009 Elsevier Inc. All rights reserved.