PVYNTN-NW, a novel recombinant strain of Potato virus Y predominating in potato fields in Syria
PVYNTN-NW, a novel recombinant strain of Potato virus Y predominating in potato fields in Syria
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DOI：
10.1111/j.1365-3059.2009.02174.x
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发表时间：
2010-02-01
期刊：
影响因子：
2.7
通讯作者：
Natsuaki, K. T.
中科院分区：
文献类型：
--
作者：
Ali, M. Chikh;Maoka, T.;Natsuaki, K. T.
对来自叙利亚的马铃薯Y病毒（PVY）重组株系PVYSYR的分离物进行了详细的鉴定，以阐明其来源，评估其意义，并最终对PVYSYR进行分类。重组分析将PVYSYR分离物分为三种重组模式，SYR-I，SYR-II和SYR-III，它们在基因组的前700个核苷酸中变化，第二种重组模式，SYR-II，最常见。PVYSYR分离株共享最高的基因组同一性和密切的系统发育关系与PVYNTN和PVYNW分离株从叙利亚，这表明一个共同的起源和当地出现的这些分离株在叙利亚。所有PVYSYR分离物（共20个）诱导烟草静脉坏死，但反应的PVYO单克隆抗体，以前报道的PVYNW（或PVYN：O）的典型特征。然而，在马铃薯中，测试的四个分离物（SYR-I中的一个和SYR-II中的三个）诱导马铃薯块茎坏死环斑病（PTNRD），这是PVYNTN的特征表型。鉴于SYR-I和SYR-II分离株与PVYNTN和PVYNW具有共同特性，因此决定它们代表PVYN菌株群的新重组菌株，拟定名称为PVYNTN-NW。SYR-III的分类只有在马铃薯中测试表型后才有可能。PVYNTN-NW在马铃薯和杂草中的高流行率以及其诱导PTNRD的能力证明了其重要性和对其进行控制的必要性。
Detailed characterization of a number of isolates of PVYSYR, a novel recombinant strain of Potato virus Y (PVY) from Syria, was conducted to elucidate their origin, assess their significance and achieve a final classification of PVYSYR. Recombination analysis grouped isolates of PVYSYR into three recombination patterns, SYR-I, SYR-II and SYR-III, which varied in the first 700 nucleotides of their genomes, with the second recombination pattern, SYR-II, the most frequent. PVYSYR isolates shared highest genomic identity and close phylogenetic relationships with PVYNTN and PVYNW isolates from Syria, suggesting a common origin and local emergence of these isolates in Syria. All PVYSYR isolates (total of 20) induced tobacco veinal necrosis, but reacted to a PVYO monoclonal antibody, typical characteristics of the previously reported PVYNW (or PVYN:O). In potato, however, four isolates tested (one of SYR-I and three of SYR-II) induced potato tuber necrotic ringspot disease (PTNRD), which is the characteristic phenotype of PVYNTN. Given the shared properties of SYR-I and SYR-II isolates with PVYNTN and PVYNW, it was decided that they represent a new recombinant strain of the PVYN strain group, with the proposed name PVYNTN-NW. The classification of SYR-III will be possible only after testing the phenotype in potato. The high prevalence of PVYNTN-NW in potatoes and weeds, as well as its ability to induce PTNRD, demonstrates its importance and the necessity for its control.