Androgen receptor functioned as a suppressor in the prostate cancer cell line PC3 in vitro and in vivo.
Androgen receptor functioned as a suppressor in the prostate cancer cell line PC3 in vitro and in vivo.
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DOI：
10.3760/cma.j.issn.0366-6999.2009.22.020
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发表时间：
2009-11
影响因子：
6.1
通讯作者：
Sheng-qiang Yu;B. Han;Y. Shao;Ji-tao Wu;Fu-jun Zhao;Hai-tao Liu;Xiao-Wen Sun;Yue-qing Tang;S. Xia
中科院分区：
文献类型：
--
作者：
Sheng-qiang Yu;B. Han;Y. Shao;Ji-tao Wu;Fu-jun Zhao;Hai-tao Liu;Xiao-Wen Sun;Yue-qing Tang;S. Xia
背景前列腺癌是世界上最常见的泌尿生殖系统肿瘤之一，在我国的发病率呈上升趋势。雄激素剥夺治疗是晚期前列腺癌的主要治疗选择。然而，雄激素受体（AR）在难治性前列腺癌中的作用仍不清楚。本研究旨在通过体内外研究探讨AR在雄激素非依赖性前列腺癌细胞系中的作用。方法采用MTT法、软琼脂法、小室侵袭法、创伤愈合法和小鼠原位移植瘤模型，观察AR对人AR基因稳定表达的PC 3-AR 9细胞增殖和侵袭转移能力的影响。结果在MTT、软琼脂、小室侵袭实验和创伤愈合实验中，雄激素受体缺失导致PC 3细胞增殖能力和侵袭转移能力下降。在小鼠原位异种移植模型中，PC 3-AR 9导致较小的原发性肿瘤和转移性肿瘤，具有较低的增殖率和较高的凋亡率。结论AR在体内外均具有抑癌作用。
BACKGROUND Prostate cancer is one of the most common urogenital tumors in the world with an increasing incidence in China. Androgen deprivation therapy is the major therapeutic option for advanced prostate cancer. However, the role of androgen receptor (AR) in hormone-refractory prostate cancer still remains unclear. This work aimed to investigate the role of AR in an androgen independent prostate cancer cell line by in vitro and in vivo studies. METHODS The role of AR in the proliferation and invasion/metastasis ability of PC3-AR9 (a PC3 stable clone expressing human AR driven by natural human AR promoter) were examined with MTT assay, soft agar assay, chamber invasion assay, wound healing assay, and also with orthotopic xenograft mouse model. RESULTS Restoring androgen receptor in PC3 cells resulted in decreased proliferation and invasion/metastasis ability in MTT, soft agar, chamber invasion and wound healing assay. In the mouse orthotopic xenograft model, PC3-AR9 resulted in smaller primary tumors and metastasis tumors, with a lower proliferation rate and higher apoptosis rate. CONCLUSION The AR might function as a tumor suppressor in PC3 cells both in vitro and in vivo.