THE EMBRYONIC GAMMA-SUBUNIT OF THE NICOTINIC ACETYLCHOLINE-RECEPTOR IS EXPRESSED IN ADULT EXTRAOCULAR-MUSCLE
THE EMBRYONIC GAMMA-SUBUNIT OF THE NICOTINIC ACETYLCHOLINE-RECEPTOR IS EXPRESSED IN ADULT EXTRAOCULAR-MUSCLE
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DOI：
10.1212/wnl.43.5.983
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发表时间：
1993-05-01
期刊：
影响因子：
9.9
通讯作者：
CONTITRONCONI, BM
中科院分区：
文献类型：
--
作者：
HORTON, RM;MANFREDI, AA;CONTITRONCONI, BM
我们用PCR方法扩增了成年牛眼外肌乙酰胆碱受体（AChR）“胚胎”γ亚基的cDNA序列。我们克隆并测序了该产物，并用它来探测北方印迹。我们在EOM mRNA中检测到γ亚基，但在对照骨骼肌中未检测到。眼外肌中γ亚单位的存在可以解释它们优先参与重症肌无力，其中通常存在对γ亚单位的自身免疫反应。
We have PCR-amplified cDNA sequence of the 'embryonic'' gamma subunit of muscle acetylcholine receptor (AChR) from adult bovine extraocular muscle (EOM). We cloned and sequenced this product and used it to probe Northern blots. We detected the gamma subunit in EOM mRNA, but not in control skeletal muscle. The presence of gamma subunit in EOMs may explain their preferential involvement in myasthenia gravis, where an autoimmune response to the gamma subunit is generally present.